3D Assessment of irreversible electroporation treatments in vegetal models

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• dc.contributor.author Castellví Fernández, Quimca
• dc.contributor.author Banús Cobo, Jaumeca
• dc.contributor.author Ivorra Cano, Antoni, 1974-ca
• dc.date.accessioned 2016-01-12T12:29:22Z
• dc.date.available 2016-01-12T12:29:22Z
• dc.date.issued 2015ca
• dc.description.abstract Ablative techniques based on irreversible elec-troporation (IRE) have emerged as an effective cancer thera-py. In these treatments electrical pulses are delivered in order to induce irrecoverable damage to the cell membranes, thus, causing cell death. An interesting feature of electroporation treatments is the capability to predict shape and extension of lesions by means of mathematical simulations. Those predic-tion methods have been refined thought in-vivo experimental observations. Aiming at minimization of animal experiments, vegetal models have been used to easily observe the IRE out-comes. However, these observations are limited at the surface of the tissue. Here we present an improved method able to observe the inner parts of the tissue and therefore evaluate in three-dimensions the results of IRE using potatoes as vegetal models. The technique consists in using a dye solution to en-hance the IRE area in sliced potato tubers. After slice digitali-zation, the electroporated area is automatically identified and the resulting treated volume is calculated. In addition, a three-dimensional reconstruction of both healthy tissue and IRE volume is generated. The proposed evaluation technique was used to assess different pulse protocols outcomes. Numerical simulations had been carried out to compare the numerical predictions to the experimental observations. The obtained results show a clear match between experimental and simulat-ed volumes confirming the reliability of the proposed method.en
• dc.description.sponsorship This work received financial support from the Spanish Government through grants TEC2010-17285 and TEC2014- 52383-C3 (TEC2014-52383-C3-2-R) and from the Europe-an Commission through the Marie Curie grant TAMIVIVE (256376).
• dc.format.mimetype application/pdfca
• dc.identifier.citation Castellví Q, Banús J, Ivorra A. 3D Assessment of irreversible electroporation treatments in vegetal models. In: Jarm T, Kramar P, editors. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies; 2015 Sept 6-10; Portorož, Slovenia. Singapore: Springer Singapore, 2015. p. 294-297. DOI 10.1007/978-981-287-817-5_65.ca
• dc.identifier.doi http://dx.doi.org/10.1007/978-981-287-817-5_65
• dc.identifier.uri http://hdl.handle.net/10230/25553
• dc.language.iso engca
• dc.publisher Springerca
• dc.relation.ispartof Jarm T, Kramar P, editors. 1st World Congress on Electroporation and Pulsed Electric Fields in Biology, Medicine and Food & Environmental Technologies; 2015 Sept 6-10; Portorož, Slovenia. Singapore: Springer Singapore, 2015. p. 294-297.
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/256376
• dc.rights © Springer (The original publication is available at www.springerlink.com)ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.subject.keyword Irreversible electroporation
• dc.subject.keyword In-vivo assessment
• dc.title 3D Assessment of irreversible electroporation treatments in vegetal modelsca
• dc.type info:eu-repo/semantics/conferenceObjectca
• dc.type.version info:eu-repo/semantics/acceptedVersionca