Comparative metagenomics reveals poultry and swine farming are hotspots for multidrug and tetracycline resistance

Osorio, Victoria; Sabater i Mezquita, Arnau; Balcázar, José Luis

doi:http://dx.doi.org/10.1016/j.envpol.2023.121239

Comparative metagenomics reveals poultry and swine farming are hotspots for multidrug and tetracycline resistance

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Osorio V, Sabater A, Balcázar JL. Comparative metagenomics reveals poultry and swine farming are hotspots for multidrug and tetracycline resistance. Environmental Pollution. 2023 Apr 1;322:121239. DOI: 10.1016/j.envpol.2023.121239

Abstract

Antibiotic misuse in livestock is a major threat to human health, as bacteria are quickly developing resistance to them. We performed a comparative analysis of 25 faecal metagenomes from swine, poultry, cattle, and humans to investigate their resistance profiles. Our analysis revealed that all genes conferring resistance to antibiotic classes assessed except tetracyclines were more prevalent in poultry manure than in the remaining species. We detected clinically relevant antibiotic resistance genes, such as mcr-1 which confers resistance to polymyxins. Among them, extended-spectrum β-lactamase blaCTX-M genes were particularly abundant in all species. Poultry manure was identified as a hotspot for multidrug resistance, which may compromise medical treatment options. Urgent actions in the livestock industry are imperative to hamper the emergence and spread of antibiotic resistance.