Electrical modeling of the influence of medium conductivity on electroporation

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• dc.contributor.author Ivorra Cano, Antoni, 1974-ca
• dc.contributor.author Villemejane, Julienca
• dc.contributor.author Mir, Lluis M.ca
• dc.date.accessioned 2016-04-21T12:15:51Z
• dc.date.available 2016-04-21T12:15:51Z
• dc.date.issued 2010ca
• dc.description.abstract Electroporation is the phenomenon in which cell membrane permeability is increased by exposing the cell to short high electric field pulses. Experimental data show that the amount of permeabilization depends on the conductivity of the extracellular medium. If medium conductivity decreases then it is necessary to deliver a pulse of larger field amplitude in order to achieve the same effect. Models that do not take into account the permeabilization effect on the membrane conductivity cannot reproduce qualitatively the experimental observations. Here we employ an exponential function for describing the strong dependence of membrane conductivity on transmembrane potential. Combining that model with numerical methods we demonstrate that the dependence on medium conductivity can be explained as being the result of increased membrane conductance due to electroporation. As experimentally observed, extracellular conductivities of about 1 and 0.1 S m−1 yield very similar results, however, for lower conductivities (<0.01 S m−1) the model predicts that significantly higher field magnitudes will be required to achieve the same amount of permeabilization.en
• dc.description.sponsorship This work was supported by grants from: CNRS, Institute of cancerology Gustave-Roussy, University Paris Sud, INCa (National Institute of Cancer, France—contract number 07/3D1616/Doc-54-3NG-NC) and French National Agency (ANR) through Nanoscience and Nanotechnology program (Nanopulsebiochip no. ANR-08-NANO-024). AI’s research is currently supported by a Ramon y Cajal/nfellowship from the Spanish Ministry for Science and Innovation.en
• dc.format.mimetype application/pdfca
• dc.identifier.citation Ivorra A, Villemejane J, Mir LM. Electrical modeling of the influence of medium conductivity on electroporation. Phys Chem Chem Phys. 2010;12(34):10055-64. DOI:10.1039/c004419aca
• dc.identifier.doi http://dx.doi.org/10.1039/c004419a
• dc.identifier.issn 1463-9076ca
• dc.identifier.uri http://hdl.handle.net/10230/26152
• dc.language.iso engca
• dc.publisher Royal Society of Chemistryca
• dc.relation.ispartof Journal of physical chemistry & biophysics. 2010;12(34):10055-64.
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TEC2014-52383-C3-2-R
• dc.rights © Royal Society of Chemistryca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.title Electrical modeling of the influence of medium conductivity on electroporationca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/acceptedVersionca