Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery

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  • dc.contributor.author Vega, Montserrat
  • dc.contributor.author Barrios, Rubén
  • dc.contributor.author Fraile Beneitez, Rodrigo, 1992-
  • dc.contributor.author Castro Cogle, Kevin de
  • dc.contributor.author Castillo Andreo, David
  • dc.contributor.author Anglada Busquets, Roger
  • dc.contributor.author Casals López, Ferran
  • dc.contributor.author Ayté del Olmo, José
  • dc.contributor.author Lowy Gallego, Ernesto
  • dc.contributor.author Hidalgo Hernando, Elena
  • dc.date.accessioned 2024-01-09T15:45:32Z
  • dc.date.available 2024-01-09T15:45:32Z
  • dc.date.issued 2023
  • dc.description.abstract Chromatin remodeling is essential to allow full development of alternative gene expression programs in response to environmental changes. In fission yeast, oxidative stress triggers massive transcriptional changes including the activation of hundreds of genes, with the participation of histone modifying complexes and chromatin remodelers. DNA transcription is associated to alterations in DNA topology, and DNA topoisomerases facilitate elongation along gene bodies. Here, we test whether the DNA topoisomerase Top1 participates in the RNA polymerase II-dependent activation of the cellular response to oxidative stress. Cells lacking Top1 are resistant to H2O2 stress. The transcriptome of Δtop1 strain was not greatly affected in the absence of stress, but activation of the anti-stress gene expression program was more sustained than in wild-type cells. Top1 associated to stress open reading frames. While the nucleosomes of stress genes are partially and transiently evicted during stress, the chromatin configuration remains open for longer times in cells lacking Top1, facilitating RNA polymerase II progression. We propose that, by removing DNA tension arising from transcription, Top1 facilitates nucleosome reassembly and works in synergy with the chromatin remodeler Hrp1 as opposing forces to transcription and to Snf22 / Hrp3 opening remodelers.
  • dc.description.sponsorship PGC2018-093920-B-I00 and PID2021-122837-NB-I00 to E.H., funded by MCIN/AEI/ 10.13039/501100011033 and by ‘ERDF A way of making Europe’; ‘European Union’. The Oxidative Stress and Cell Cycle group is also supported by Generalitat de Catalunya (Spain) [2017-SGR-539 and 2021-SGR-00007]; Excellence Unit «María de Maeztu» Grant CEX2018-000792-M funded by MCIN/AEI /10.13039/501100011033; E.H. is recipient of an ICREA Academia Award (Generalitat de Catalunya, Spain); R.F. was recipient of a FPI fellowship from the Ministerio de Economía y Competitividad (Spain). Funding for open access charge: Ministerio de Ciencia e Innovación, Gobierno de España [PID2021-122837-NB-I00].
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Vega M, Barrios R, Fraile R, de Castro Cogle K, Castillo D, Anglada R, Casals F, Ayté J, Lowy-Gallego E, Hidalgo E. Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery. Nucleic Acids Res. 2023 Dec 11;51(22):12161-73. DOI: 10.1093/nar/gkad1066
  • dc.identifier.doi http://dx.doi.org/10.1093/nar/gkad1066
  • dc.identifier.issn 0305-1048
  • dc.identifier.uri http://hdl.handle.net/10230/58659
  • dc.language.iso eng
  • dc.publisher Oxford University Press
  • dc.relation.ispartof Nucleic Acids Res. 2023 Dec 11;51(22):12161-73
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PGC2018-093920-B-I00
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-122837-NB-I00
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/3PE/PID2021-122837-NB-I00
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
  • dc.rights © The Author(s) 2023. Published by Oxford University Press on behalf of Nucleic Acids Research. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (https://creativecommons.org/licenses/by/4.0/), which permits unrestricted reuse, distribution, and reproduction in any medium, provided the original work is properly cited.
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by/4.0/
  • dc.subject.keyword Gene Regulation
  • dc.subject.keyword Chromatin and Epigenetics
  • dc.title Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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