Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery

Vega, Montserrat; Barrios Fernández, Rubén; Fraile Beneitez, Rodrigo, 1992-; Castro Cogle, Kevin de; Castillo Andreo, David; Anglada Busquets, Roger; Casals López, Ferran; Ayté del Olmo, José; Lowy Gallego, Ernesto; Hidalgo Hernando, Elena

doi:http://dx.doi.org/10.1093/nar/gkad1066

Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery

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Vega M, Barrios R, Fraile R, de Castro Cogle K, Castillo D, Anglada R, Casals F, Ayté J, Lowy-Gallego E, Hidalgo E. Topoisomerase 1 facilitates nucleosome reassembly at stress genes during recovery. Nucleic Acids Res. 2023 Dec 11;51(22):12161-73. DOI: 10.1093/nar/gkad1066

Abstract

Chromatin remodeling is essential to allow full development of alternative gene expression programs in response to environmental changes. In fission yeast, oxidative stress triggers massive transcriptional changes including the activation of hundreds of genes, with the participation of histone modifying complexes and chromatin remodelers. DNA transcription is associated to alterations in DNA topology, and DNA topoisomerases facilitate elongation along gene bodies. Here, we test whether the DNA topoisomerase Top1 participates in the RNA polymerase II-dependent activation of the cellular response to oxidative stress. Cells lacking Top1 are resistant to H2O2 stress. The transcriptome of Δtop1 strain was not greatly affected in the absence of stress, but activation of the anti-stress gene expression program was more sustained than in wild-type cells. Top1 associated to stress open reading frames. While the nucleosomes of stress genes are partially and transiently evicted during stress, the chromatin configuration remains open for longer times in cells lacking Top1, facilitating RNA polymerase II progression. We propose that, by removing DNA tension arising from transcription, Top1 facilitates nucleosome reassembly and works in synergy with the chromatin remodeler Hrp1 as opposing forces to transcription and to Snf22 / Hrp3 opening remodelers.