Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: a proof of concept

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• dc.contributor.author Gomez-Gonzalez, Emilio
• dc.contributor.author Gómez Gutiérrez, Emilia, 1975-
• dc.contributor.author García-Cózar, Francisco J.
• dc.contributor.author Marquez-Rivas, Javier
• dc.date.accessioned 2025-04-22T06:54:12Z
• dc.date.available 2025-04-22T06:54:12Z
• dc.date.issued 2023
• dc.description Includes supplementary materials for the online appendix.
• dc.description.abstract Objective. To conduct a proof-of-concept study of the detection of two synthetic models of severe acute respiratory syndrome coronavirus 2 (SARS-CoV-2) using polarimetric imaging. Approach. Two SARS-CoV-2 models were prepared as engineered lentiviruses pseudotyped with the G protein of the vesicular stomatitis virus, and with the characteristic Spike protein of SARS-CoV-2. Samples were prepared in two biofluids (saline solution and artificial saliva), in four concentrations, and deposited as 5-µL droplets on a supporting plate. The angles of maximal degree of linear polarization (DLP) of light diffusely scattered from dry residues were determined using Mueller polarimetry from87 samples at 405 nm and 514 nm. A polarimetric camera was used for imaging several samples under 380–420 nm illumination at angles similar to those of maximal DLP. Per-pixel image analysis included quantification and combination of polarization feature descriptors in 475 samples. Main results. The angles (from sample surface) of maximal DLP were 3° for 405 nm and 6° for 514 nm. Similar viral particles that differed only in the characteristic spike protein of the SARS-CoV-2, their corresponding negative controls, fluids, and the sample holder were discerned at 10-degree and 15-degree configurations. Significance. Polarimetric imaging in the visible spectrum may help improve fast, non-contact detection and identification of viral particles, and/or other microbes such as tuberculosis, in multiple dry fluid samples simultaneously, particularly when combined with other imaging modalities. Further analysis including realistic concentrations of real SARS-CoV-2 viral particles in relevant human fluids is required. Polarimetric imaging under visible light may contribute to a fast, cost-effective screening of SARS-CoV-2 and other pathogens when combined with other imaging modalities.en
• dc.description.sponsorship This study was funded by grants number COV20–00080 and COV20–00173 of the 2020 Emergency Call for Research Projects about the SARS-CoV-2 virus and the COVID-19 disease of the Institute of Health ‘Carlos III’, Spanish Ministry of Science and Innovation, and by grant number EQC2019–006240-P funded by MICIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. ABR was supported by grant number RTI2018–094465-J-I00 funded by MICIN/AEI/10.13039/501100011033 and by “ERDF A way of making Europe”. The work of OM, JCGM, and JLR has been partially funded by grant LEONIDAS (RTI2018–095330-B-100), and the Center of Excellence Severo Ochoa award to the Instituto de Astrofísica de Andalucía (SEV-2017–0709). This work has been supported by the European Commission through the Joint Research Center (JRC) HUMAINT project. The authors would like to gratefully acknowledge the assistance of the members of the Explosive Ordnance Disposal – Chemical, Biological, Radiological & Nuclear (EOD-CBRN) Group of the Spanish National Police, whose identities cannot be disclosed, and who are represented here by JMNG. The authors would like to express their special gratitude to Emilio Gomez-Gonzalez, who sadly passed away in January 2023, and whose wisdom and willingness have been key in the development of this work and many other projects.en
• dc.format.mimetype application/pdf
• dc.identifier.citation Gomez-Gonzalez E, Muñoz O, Gomez-Martin JC, Aceituno-Castro J, Fernandez-Muñoz B, Navas-Garcia JM, et al. Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: a proof of concept. J Quant Spectrosc Radiat Transf. 2023 Jul;302:108567. DOI: 10.1016/j.jqsrt.2023.108567
• dc.identifier.doi http://dx.doi.org/10.1016/j.jqsrt.2023.108567
• dc.identifier.issn 0022-4073
• dc.identifier.uri http://hdl.handle.net/10230/70167
• dc.language.iso eng
• dc.publisher Elsevier
• dc.relation.ispartof Journal of Quantitative Spectroscopy and Radiative Transfer. 2023 Jul;302:108567
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RTI2018–094465-J-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/RTI2018–095330-B-I00
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/SEV-2017–0709
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/EQC2019-006240-P
• dc.rights © 2023 The Author(s). Published by Elsevier Ltd. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.subject.keyword COVID-19en
• dc.subject.keyword Mueller polarimetryen
• dc.subject.keyword Polarimetric imagingen
• dc.subject.keyword SARS-CoV-2en
• dc.subject.keyword Virus detectionen
• dc.title Polarimetric imaging for the detection of synthetic models of SARS-CoV-2: a proof of concepten
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion