A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk

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• dc.contributor.author Steck, Kathrinca
• dc.contributor.author Veit, Danielca
• dc.contributor.author Grandy, Ronaldca
• dc.contributor.author Bermúdez i Badia, Sergica
• dc.contributor.author Mathews, Zenonca
• dc.contributor.author Verschure, Paul F. M. J.ca
• dc.contributor.author Hansson, B. S. (Bill S.), 1959-ca
• dc.contributor.author Knaden, Markusca
• dc.date.accessioned 2016-02-18T18:47:42Z
• dc.date.available 2016-02-18T18:47:42Z
• dc.date.issued 2012
• dc.description.abstract How can odor-guided behavior of numerous individual Drosophila be assessed automatically with high temporal resolution? For this purpose we introduce the automatic integrated tracking and odor-delivery system Flywalk. In fifteen aligned small wind tunnels individual flies are exposed to repeated odor pulses, well defined in concentration and timing. The flies' positions are visually tracked, which allows quantification of the odor-evoked walking behavior with high temporal resolution of up to 100 ms. As a demonstration of Flywalk we show that the flies' behavior is odorant-specific; attractive odors elicit directed upwind movements, while repellent odors evoke decreased activity, followed by downwind movements. These changes in behavior differ between sexes. Furthermore our findings show that flies can evaluate the sex of a conspecific and males can determine a female's mating status based on olfactory cues. Consequently, Flywalk allows automatic screening of individual flies for their olfactory preference and sensitivity.ca
• dc.description.sponsorship This study was supported by fundings from the Max Planck Society to BSH.
• dc.format.mimetype application/pdfca
• dc.identifier.citation Steck K, Veit D, Grandy R, Badia SBI, Mathews Z, Verschure P, Hansson BS, Knaden M. A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalk. Scientific Reports. 2012;2(361). DOI: 10.1038/srep00361.
• dc.identifier.doi http://dx.doi.org/10.1038/srep00361
• dc.identifier.issn 2045-2322
• dc.identifier.uri http://hdl.handle.net/10230/25901
• dc.language.iso engca
• dc.publisher Nature Publishing Groupca
• dc.relation.ispartof Scientific Reports. 2012;2(361)
• dc.rights This work is licensed under a Creative Commons Attribution-NonCommercial-ShareALike 3.0 Unported License. To view a copy of this license, visit http://creativecommons.org/licenses/by-nc-sa/3.0/ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by-nc-sa/3.0/ca
• dc.subject.keyword Animal behaviour
• dc.subject.keyword Biological models
• dc.subject.keyword Olfactory system
• dc.title A high-throughput behavioral paradigm for Drosophila olfaction - The Flywalkca
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca