Chromatin-bound proteome profiling by genome capture
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- dc.contributor.author Aranda Aragón, Sergio
- dc.contributor.author Borràs, Eva
- dc.contributor.author Sabidó Aguadé, Eduard, 1981-
- dc.contributor.author Di Croce, Luciano
- dc.date.accessioned 2020-11-18T07:04:44Z
- dc.date.available 2020-11-18T07:04:44Z
- dc.date.issued 2020
- dc.description.abstract De novo identification of chromatin interactors can reveal unexpected pathways relevant to physiology and human disease. Inspired by the DNA mediated chromatin pull-down (Dm-ChP) technology (also known as iPOND [isolation of proteins on nascent DNA]) for the proteomic characterization of nascent DNA, we have recently reported a new experimental protocol that allows for the identification of proteins on total DNA (iPOTD) for bulk chromatome profiling and de novo identification of chromatin-bound proteins. Here, we detail a step-by-step protocol to survey the cellular chromatin-bound proteome in a simple, robust, and unbiased manner. For complete details on the use and execution of this protocol, please refer to Aranda et al. (2019).
- dc.description.sponsorship The Proteomics Unit is supported by EPIC-XS, project number 823839, funded by the Horizon 2020 programme of the European Union. The Di Croce Laboratory is supported by grants from the Spanish Ministerio de Educación y Ciencia (BFU2016-75008-P) and by AGAUR.
- dc.format.mimetype application/pdf
- dc.identifier.citation Aranda S, Borràs E, Sabidó E, Di Croce L. Chromatin-bound proteome profiling by genome capture. STAR Protoc. 2020; 1(1):100014. DOI: 10.1016/j.xpro.2020.100014
- dc.identifier.doi http://dx.doi.org/10.1016/j.xpro.2020.100014
- dc.identifier.issn 2666-1667
- dc.identifier.uri http://hdl.handle.net/10230/45793
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof STAR Protoc. 2020; 1(1):100014
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/823839
- dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2016-75008-P
- dc.rights © 2020. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.title Chromatin-bound proteome profiling by genome capture
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion