Chromatin-bound proteome profiling by genome capture

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dc.contributor.authorAranda Aragón, Sergio
dc.contributor.authorBorràs, Eva
dc.contributor.authorSabidó Aguadé, Eduard, 1981-
dc.contributor.authorDi Croce, Luciano
dc.date.accessioned2020-11-18T07:04:44Z
dc.date.available2020-11-18T07:04:44Z
dc.date.issued2020
dc.description.abstractDe novo identification of chromatin interactors can reveal unexpected pathways relevant to physiology and human disease. Inspired by the DNA mediated chromatin pull-down (Dm-ChP) technology (also known as iPOND [isolation of proteins on nascent DNA]) for the proteomic characterization of nascent DNA, we have recently reported a new experimental protocol that allows for the identification of proteins on total DNA (iPOTD) for bulk chromatome profiling and de novo identification of chromatin-bound proteins. Here, we detail a step-by-step protocol to survey the cellular chromatin-bound proteome in a simple, robust, and unbiased manner. For complete details on the use and execution of this protocol, please refer to Aranda et al. (2019).
dc.description.sponsorshipThe Proteomics Unit is supported by EPIC-XS, project number 823839, funded by the Horizon 2020 programme of the European Union. The Di Croce Laboratory is supported by grants from the Spanish Ministerio de Educación y Ciencia (BFU2016-75008-P) and by AGAUR.
dc.format.mimetypeapplication/pdf
dc.identifier.citationAranda S, Borràs E, Sabidó E, Di Croce L. Chromatin-bound proteome profiling by genome capture. STAR Protoc. 2020; 1(1):100014. DOI: 10.1016/j.xpro.2020.100014
dc.identifier.doihttp://dx.doi.org/10.1016/j.xpro.2020.100014
dc.identifier.issn2666-1667
dc.identifier.urihttp://hdl.handle.net/10230/45793
dc.language.isoeng
dc.publisherElsevier
dc.relation.ispartofSTAR Protoc. 2020; 1(1):100014
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/823839
dc.relation.projectIDinfo:eu-repo/grantAgreement/ES/1PE/BFU2016-75008-P
dc.rights© 2020. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by-nc-nd/4.0/
dc.titleChromatin-bound proteome profiling by genome capture
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion

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