Chromatin-bound proteome profiling by genome capture
Chromatin-bound proteome profiling by genome capture
Citació
- Aranda S, Borràs E, Sabidó E, Di Croce L. Chromatin-bound proteome profiling by genome capture. STAR Protoc. 2020; 1(1):100014. DOI: 10.1016/j.xpro.2020.100014
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Descripció
Resum
De novo identification of chromatin interactors can reveal unexpected pathways relevant to physiology and human disease. Inspired by the DNA mediated chromatin pull-down (Dm-ChP) technology (also known as iPOND [isolation of proteins on nascent DNA]) for the proteomic characterization of nascent DNA, we have recently reported a new experimental protocol that allows for the identification of proteins on total DNA (iPOTD) for bulk chromatome profiling and de novo identification of chromatin-bound proteins. Here, we detail a step-by-step protocol to survey the cellular chromatin-bound proteome in a simple, robust, and unbiased manner. For complete details on the use and execution of this protocol, please refer to Aranda et al. (2019).