Circadian transcriptome processing and analysis: a workflow for muscle stem cells

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dc.contributor.authorSica, Valentina
dc.contributor.authorDeryagin, Oleg
dc.contributor.authorSmith, Jacob G.
dc.contributor.authorMuñoz Cánoves, Pura, 1962-
dc.date.accessioned2023-06-14T13:47:33Z
dc.date.available2023-06-14T13:47:33Z
dc.date.issued2023
dc.description.abstractCircadian rhythms coordinate biological processes with Earth's 24-h daily light/dark cycle. In the last years, efforts in the field of chronobiology have sought to understand the ways in which the circadian clock controls transcription across tissues and cells. This has been supported by the development of different bioinformatic approaches that allow the identification of 24-h oscillating transcripts. This workflow aims to describe how to isolate muscle stem cells for RNA sequencing analysis from a typical circadian experiment and introduces bioinformatic tools suitable for the analysis of circadian transcriptomes.
dc.description.sponsorshipH2020 Marie Skłodowska-Curie Actions. Grant Number: 895380
dc.format.mimetypeapplication/pdf
dc.identifier.citationSica V, Deryagin O, Smith, JG, Muñoz-Canoves P. Circadian transcriptome processing and analysis: a workflow for muscle stem cells. FEBS Open Bio. 2023 May 12. DOI: 10.1002/2211-5463.13629
dc.identifier.doihttp://dx.doi.org/10.1002/2211-5463.13629
dc.identifier.issn2211-5463
dc.identifier.urihttp://hdl.handle.net/10230/57166
dc.language.isoeng
dc.publisherWiley
dc.relation.ispartofFEBS Open Bio. 2023 May 12
dc.relation.projectIDinfo:eu-repo/grantAgreement/EC/H2020/895380
dc.rights© 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
dc.rights.accessRightsinfo:eu-repo/semantics/openAccess
dc.rights.urihttp://creativecommons.org/licenses/by/4.0/
dc.subject.keywordBioinformatics
dc.subject.keywordCircadian rhythms
dc.subject.keywordMuscle stem cells
dc.subject.keywordSatellite cells
dc.titleCircadian transcriptome processing and analysis: a workflow for muscle stem cells
dc.typeinfo:eu-repo/semantics/article
dc.type.versioninfo:eu-repo/semantics/publishedVersion

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