Circadian transcriptome processing and analysis: a workflow for muscle stem cells
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- dc.contributor.author Sica, Valentina
- dc.contributor.author Deryagin, Oleg
- dc.contributor.author Smith, Jacob G.
- dc.contributor.author Muñoz Cánoves, Pura, 1962-
- dc.date.accessioned 2023-06-14T13:47:33Z
- dc.date.available 2023-06-14T13:47:33Z
- dc.date.issued 2023
- dc.description.abstract Circadian rhythms coordinate biological processes with Earth's 24-h daily light/dark cycle. In the last years, efforts in the field of chronobiology have sought to understand the ways in which the circadian clock controls transcription across tissues and cells. This has been supported by the development of different bioinformatic approaches that allow the identification of 24-h oscillating transcripts. This workflow aims to describe how to isolate muscle stem cells for RNA sequencing analysis from a typical circadian experiment and introduces bioinformatic tools suitable for the analysis of circadian transcriptomes.
- dc.description.sponsorship H2020 Marie Skłodowska-Curie Actions. Grant Number: 895380
- dc.format.mimetype application/pdf
- dc.identifier.citation Sica V, Deryagin O, Smith, JG, Muñoz-Canoves P. Circadian transcriptome processing and analysis: a workflow for muscle stem cells. FEBS Open Bio. 2023 May 12. DOI: 10.1002/2211-5463.13629
- dc.identifier.doi http://dx.doi.org/10.1002/2211-5463.13629
- dc.identifier.issn 2211-5463
- dc.identifier.uri http://hdl.handle.net/10230/57166
- dc.language.iso eng
- dc.publisher Wiley
- dc.relation.ispartof FEBS Open Bio. 2023 May 12
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/895380
- dc.rights © 2023 The Authors. FEBS Open Bio published by John Wiley & Sons Ltd on behalf of Federation of European Biochemical Societies. This is an open access article under the terms of the Creative Commons Attribution License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited.
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword Bioinformatics
- dc.subject.keyword Circadian rhythms
- dc.subject.keyword Muscle stem cells
- dc.subject.keyword Satellite cells
- dc.title Circadian transcriptome processing and analysis: a workflow for muscle stem cells
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion