Circadian transcriptome processing and analysis: a workflow for muscle stem cells

Sica, Valentina; Deryagin, Oleg; Smith, Jacob G.; Muñoz Cánoves, Pura, 1962-

doi:http://dx.doi.org/10.1002/2211-5463.13629

Circadian transcriptome processing and analysis: a workflow for muscle stem cells

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Sica V, Deryagin O, Smith, JG, Muñoz-Canoves P. Circadian transcriptome processing and analysis: a workflow for muscle stem cells. FEBS Open Bio. 2023 May 12. DOI: 10.1002/2211-5463.13629

Abstract

Circadian rhythms coordinate biological processes with Earth's 24-h daily light/dark cycle. In the last years, efforts in the field of chronobiology have sought to understand the ways in which the circadian clock controls transcription across tissues and cells. This has been supported by the development of different bioinformatic approaches that allow the identification of 24-h oscillating transcripts. This workflow aims to describe how to isolate muscle stem cells for RNA sequencing analysis from a typical circadian experiment and introduces bioinformatic tools suitable for the analysis of circadian transcriptomes.