Acid-modulated peptide synthesis for application on oxide biosensor interfaces
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- dc.contributor.author Cristóbal-Lecina, Edgar
- dc.contributor.author El-Maiss, Janwa
- dc.contributor.author Figueras, Eduard
- dc.contributor.author Singh, Aruna Chandra
- dc.contributor.author Krishnamoorthy, Sivashankar
- dc.contributor.author Osterbye, Thomas
- dc.contributor.author Pascual García, César
- dc.contributor.author Andreu Martínez, David
- dc.date.accessioned 2024-02-06T07:24:43Z
- dc.date.available 2024-02-06T07:24:43Z
- dc.date.issued 2023
- dc.description.abstract In this paper we report an acid-modulated strategy for novel peptide microarray production on biosensor interfaces. We initially selected a controlled pore glass (CPG) as a support for solid-phase peptide synthesis (SPPS) to implement a chemistry that can be performed at the interface of multiple field effect transistor (FET) sensors, eventually to generate label-free peptide microarrays for protein screening. Our chemistry uses a temporary protection of the N-terminal amino function of each amino acid building block with a tert-butyloxycarbonyl (Boc) group that can be removed after each SPPS cycle, in combination with semi-permanent protection of the side chains of trifunctional amino acid residues. Such a protection scheme with a well-proven record of application in conventional, batchwise SPPS has been fine-tuned for optimal performance on CPG and, from there, translated to SPR chips that allow layer-by-layer monitoring of amino acid coupling. Our results validate this acid-modulated synthesis as a feasible approach for producing peptides in high yields and purity on flat glass surfaces, such as those in bio-FETs.
- dc.description.sponsorship This project was funded by the FET-OPEN program of the European Innovation Council, Grant Number 862539.
- dc.format.mimetype application/pdf
- dc.identifier.citation Cristóbal-Lecina E, El-Maiss J, Figueras E, Singh AC, Krishnamoorthy S, Østerbye T, Pascual García C, Andreu D. Acid-Modulated peptide synthesis for application on oxide biosensor interfaces. Nanomaterials (Basel). 2023 Dec 6;13(24):3092. DOI: 10.3390/nano13243092
- dc.identifier.doi http://dx.doi.org/10.3390/nano13243092
- dc.identifier.issn 2079-4991
- dc.identifier.uri http://hdl.handle.net/10230/58959
- dc.language.iso eng
- dc.publisher MDPI
- dc.relation.ispartof Nanomaterials (Basel). 2023 Dec 6;13(24):3092
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/862539
- dc.rights © 2023 by the authors. Licensee MDPI, Basel, Switzerland. This article is an open access article distributed under the terms and conditions of the Creative Commons Attribution (CC BY) license (https://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword SPR chips
- dc.subject.keyword Acid-labile protection scheme
- dc.subject.keyword Glass surfaces
- dc.subject.keyword Peptide synthesis
- dc.title Acid-modulated peptide synthesis for application on oxide biosensor interfaces
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion