Acid-modulated peptide synthesis for application on oxide biosensor interfaces

Cristóbal-Lecina, Edgar; El-Maiss, Janwa; Figueras, Eduard; Singh, Aruna Chandra; Krishnamoorthy, Sivashankar; Osterbye, Thomas; Pascual García, César; Andreu Martínez, David

doi:http://dx.doi.org/10.3390/nano13243092

Acid-modulated peptide synthesis for application on oxide biosensor interfaces

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Cristóbal-Lecina E, El-Maiss J, Figueras E, Singh AC, Krishnamoorthy S, Østerbye T, Pascual García C, Andreu D. Acid-Modulated peptide synthesis for application on oxide biosensor interfaces. Nanomaterials (Basel). 2023 Dec 6;13(24):3092. DOI: 10.3390/nano13243092

Abstract

In this paper we report an acid-modulated strategy for novel peptide microarray production on biosensor interfaces. We initially selected a controlled pore glass (CPG) as a support for solid-phase peptide synthesis (SPPS) to implement a chemistry that can be performed at the interface of multiple field effect transistor (FET) sensors, eventually to generate label-free peptide microarrays for protein screening. Our chemistry uses a temporary protection of the N-terminal amino function of each amino acid building block with a tert-butyloxycarbonyl (Boc) group that can be removed after each SPPS cycle, in combination with semi-permanent protection of the side chains of trifunctional amino acid residues. Such a protection scheme with a well-proven record of application in conventional, batchwise SPPS has been fine-tuned for optimal performance on CPG and, from there, translated to SPR chips that allow layer-by-layer monitoring of amino acid coupling. Our results validate this acid-modulated synthesis as a feasible approach for producing peptides in high yields and purity on flat glass surfaces, such as those in bio-FETs.