Comprehensive analysis of animal models of cardiovascular disease using multiscale X-Ray phase contrast tomography

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• dc.contributor.author Dejea i Velardo, Hèctor
• dc.contributor.author Garcia Canadilla, Patricia
• dc.contributor.author Cook, Andrew C.
• dc.contributor.author Guasch, Eduard
• dc.contributor.author Zamora, Monica
• dc.contributor.author Crispi Brillas, Fàtima
• dc.contributor.author Stampanoni, Marco
• dc.contributor.author Bijnens, Bart
• dc.contributor.author Bonnin, Anne
• dc.date.accessioned 2021-03-30T07:57:33Z
• dc.date.available 2021-03-30T07:57:33Z
• dc.date.issued 2019
• dc.description.abstract Cardiovascular diseases (CVDs) affect the myocardium and vasculature, inducing remodelling of the heart from cellular to whole organ level. To assess their impact at micro and macroscopic level, multi-resolution imaging techniques that provide high quality images without sample alteration and in 3D are necessary: requirements not fulfilled by most of current methods. In this paper, we take advantage of the non-destructive time-efficient 3D multiscale capabilities of synchrotron Propagation-based X-Ray Phase Contrast Imaging (PB-X-PCI) to study a wide range of cardiac tissue characteristics in one healthy and three different diseased rat models. With a dedicated image processing pipeline, PB-X-PCI images are analysed in order to show its capability to assess different cardiac tissue components at both macroscopic and microscopic levels. The presented technique evaluates in detail the overall cardiac morphology, myocyte aggregate orientation, vasculature changes, fibrosis formation and nearly single cell arrangement. Our results agree with conventional histology and literature. This study demonstrates that synchrotron PB-X-PCI, combined with image processing tools, is a powerful technique for multi-resolution structural investigation of the heart ex-vivo. Therefore, the proposed approach can improve the understanding of the multiscale remodelling processes occurring in CVDs, and the comprehensive and fast assessment of future interventional approaches.
• dc.description.sponsorship We acknowledge the Paul Scherrer Institut, Villigen, Switzerland for provision of synchrotron radiation beamtime at beamline TOMCAT of the SLS, as well as the support of the Scientific Center for Optical and Electron Microscopy ScopeM of the Swiss Federal Institute of Technology ETHZ. Tis project was supported by the grant #2017‐303 of the Strategic Focal Area “Personalized Health and Related Technologies (PHRT)” of the ETH Domain, the Spanish Ministry of Economy and Competitiveness (grant TIN2014-52923-R, the Maria de Maeztu Units of Excellence Programme MDM-2015-0502) and Fondo Europeo de Desarrollo Regional (FEDER). PGC wants to acknowledge the European Molecular Biology Organization (EMBO) for her short-term grant to do a research stay at TOMCAT (PSI, Villigen, Switzerland) and to CA15124 (NEUBIAS) support for her STMS grant.
• dc.format.mimetype application/pdf
• dc.identifier.citation Dejea H, Garcia-Canadilla P, Cook AC, Guasch E, Zamora M, Crispi F, Stampanoni M, Bijnens B, Bonnin A. Comprehensive analysis of animal models of cardiovascular disease using multiscale X-Ray phase contrast tomography. Sci Rep. 2019 May 6;9:6996. DOI: 10.1038/s41598-019-43407-z
• dc.identifier.doi http://dx.doi.org/10.1038/s41598-019-43407-z
• dc.identifier.issn 2045-2322
• dc.identifier.uri http://hdl.handle.net/10230/46981
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Scientific Reports. 2019 May 6;9:6996
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TIN2014-52923-R
• dc.rights © The Author(s) 2019. This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons license, and indicate if changes were made. Te images or other third party material in this article are included in the article’s Creative Commons license, unless indicated otherwise in a credit line to the material. If material is not included in the article’s Creative Commons license and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this license, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Biological physics
• dc.subject.keyword Biomedical engineering
• dc.subject.keyword Cardiovascular diseases
• dc.subject.keyword Image processing
• dc.title Comprehensive analysis of animal models of cardiovascular disease using multiscale X-Ray phase contrast tomography
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion