Targeting human urinary metabolome by LC-MS/MS: a review

Rodríguez-Morató, Jose, 1987-; Pozo Mendoza, Óscar J., 1975-; Marcos del Águila, Josep, 1971-

doi:http://dx.doi.org/10.4155/bio-2017-0285

Targeting human urinary metabolome by LC-MS/MS: a review

Citation

Rodríguez-Morató J, Pozo ÓJ, Marcos J. Targeting human urinary metabolome by LC-MS/MS: a review. Bioanalysis. 2018 Apr 1;10(7):489-516. DOI: 10.4155/bio-2017-0285

Abstract

Urine is a biological matrix that contains hundreds of metabolic end products which constitute the urinary metabolome. The development and advances on LC-MS/MS have revolutionized the analytical study of biomolecules by enabling their accurate identification and quantification in an unprecedented manner. Nowadays, LC-MS/MS is helping to unveil the complexity of urine metabolome, and the results obtained have multiple biomedical applications. This review focuses on the targeted LC-MS/MS analysis of the urine metabolome. In the first part, we describe general considerations (from sample collection to quantitation) required for a proper targeted metabolic analysis. In the second part, we address the urinary analysis and recent applications of four relevant families: amino acids, catecholamines, lipids and steroids.