Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes.

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• dc.contributor.author Martínez Cruz, Begoñaca
• dc.contributor.author Ziegle, Janetca
• dc.contributor.author Sanz, Paulaca
• dc.contributor.author Sotelo, Gracielaca
• dc.contributor.author Anglada Busquets, Rogerca
• dc.contributor.author Plaza, Stéphanieca
• dc.contributor.author Comas, David, 1969-ca
• dc.date.accessioned 2014-12-22T13:00:36Z
• dc.date.available 2014-12-22T13:00:36Z
• dc.date.issued 2011ca
• dc.description.abstract Background: The analysis of human Y-chromosome variation in the context of population genetics and forensics requires the genotyping of dozens to hundreds of selected single-nucleotide polymorphisms (SNPs). In the present study, we developed a 121-plex (121 SNPs in a single array) TaqMan array capable of distinguishing most haplogroups and subhaplogroups on the Y-chromosome human phylogeny in Europe./nResults: We present data from 264 samples from several European areas and ethnic groups. The array developed in this study shows >99% accuracy of assignation to the Y human phylogeny (with an average call rate of genotypes >96%)./nConclusions: We have created and evaluated a robust and accurate Y-chromosome multiplex which minimises the possible errors due to mixup when typing the same sample in several independent reactions.
• dc.description.sponsorship The present study was supported by the Ministerio de Ciencia e Innovación (Madrid, Spain) (CGL2010-14944) and Direcció General de Recerca, Generalitat de Catalunya (Barcelona, Spain) (2009SGR1101).
• dc.format.mimetype application/pdfca
• dc.identifier.citation Martínez-Cruz B, Ziegle J, Sanz P, Sotelo G, Anglada R, Plaza S, Comas D. Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes. Investigative Genetics. 2011 May;2:13. DOI: 10.1186/2041-2223-2-13ca
• dc.identifier.doi http://dx.doi.org/10.1186/2041-2223-2-13
• dc.identifier.issn 2041-2223ca
• dc.identifier.uri http://hdl.handle.net/10230/23033
• dc.language.iso engca
• dc.publisher BioMed Centralca
• dc.relation.ispartof Investigative Genetics. 2011 May;2:13
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/CGL2010-14944
• dc.rights © 2011 Martínez-Cruz et al; licensee BioMed Central Ltd. /nThis is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/2.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.ca
• dc.rights.accessRights info:eu-repo/semantics/openAccessca
• dc.rights.uri http://creativecommons.org/licenses/by/2.0
• dc.subject.other Cromosoma X
• dc.subject.other Cromosoma Y
• dc.subject.other Genòmica
• dc.subject.other Filogènia
• dc.title Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes.en
• dc.type info:eu-repo/semantics/articleca
• dc.type.version info:eu-repo/semantics/publishedVersionca