Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes.
Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes.
Citació
- Martínez-Cruz B, Ziegle J, Sanz P, Sotelo G, Anglada R, Plaza S, Comas D. Multiplex single-nucleotide polymorphism typing of the human Y chromosome using TaqMan probes. Investigative Genetics. 2011 May;2:13. DOI: 10.1186/2041-2223-2-13
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Descripció
Resum
Background: The analysis of human Y-chromosome variation in the context of population genetics and forensics requires the genotyping of dozens to hundreds of selected single-nucleotide polymorphisms (SNPs). In the present study, we developed a 121-plex (121 SNPs in a single array) TaqMan array capable of distinguishing most haplogroups and subhaplogroups on the Y-chromosome human phylogeny in Europe./nResults: We present data from 264 samples from several European areas and ethnic groups. The array developed in this study shows >99% accuracy of assignation to the Y human phylogeny (with an average call rate of genotypes >96%)./nConclusions: We have created and evaluated a robust and accurate Y-chromosome multiplex which minimises the possible errors due to mixup when typing the same sample in several independent reactions.