Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector

Rodríguez Pizà, Ignasi; Barragan, Montserrat; González, Federico; Izpisúa Belmonte, J. C.; Batlle Morera, Laura; Vassena, Rita; Montserrat Pulido, Núria; Tiscornia, Gustavo

doi:http://dx.doi.org/10.1073/pnas.0901471106

Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector

Citation

González F, Barragan M, Tiscornia G, Montserrat N, Vassena R, Batlle L et al. Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector. Proc Natl Acad Sci U S A. 2009;106(22):8918-22. DOI: 10.1073/pnas.0901471106

Abstract

Induced pluripotent stem (iPS) cells have generated keen interest/ndue to their potential use in regenerative medicine. They have/nbeen obtained from various cell types of both mice and humans by/nexogenous delivery of different combinations of Oct4, Sox2, Klf4,/nc-Myc, Nanog, and Lin28. The delivery of these transcription factors/nhas mostly entailed the use of integrating viral vectors (retroviruses/nor lentiviruses), carrying the risk of both insertional mutagenesis/nand oncogenesis due to misexpression of these exogenous/nfactors. Therefore, obtaining iPS cells that do not carry integrated/ntransgene sequences is an important prerequisite for their eventual/ntherapeutic use. Here we report the generation of iPS cell lines/nfrom mouse embryonic fibroblasts with no evidence of integration/nof the reprogramming vector in their genome, achieved by nucleofection/nof a polycistronic construct coexpressing Oct4, Sox2, Klf4,/nand c-Myc