Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector
| dc.contributor.author | Rodríguez Pizà, Ignasi | ca |
| dc.contributor.author | Barragan, Montserrat | ca |
| dc.contributor.author | González, Federico | ca |
| dc.contributor.author | Izpisúa Belmonte, J. C. | ca |
| dc.contributor.author | Batlle Morera, Laura | ca |
| dc.contributor.author | Vassena, Rita | ca |
| dc.contributor.author | Montserrat Pulido, Núria | ca |
| dc.contributor.author | Tiscornia, Gustavo | ca |
| dc.date.accessioned | 2011-07-27T08:27:59Z | |
| dc.date.available | 2011-07-27T08:27:59Z | |
| dc.date.issued | 2009 | ca |
| dc.description.abstract | Induced pluripotent stem (iPS) cells have generated keen interest/ndue to their potential use in regenerative medicine. They have/nbeen obtained from various cell types of both mice and humans by/nexogenous delivery of different combinations of Oct4, Sox2, Klf4,/nc-Myc, Nanog, and Lin28. The delivery of these transcription factors/nhas mostly entailed the use of integrating viral vectors (retroviruses/nor lentiviruses), carrying the risk of both insertional mutagenesis/nand oncogenesis due to misexpression of these exogenous/nfactors. Therefore, obtaining iPS cells that do not carry integrated/ntransgene sequences is an important prerequisite for their eventual/ntherapeutic use. Here we report the generation of iPS cell lines/nfrom mouse embryonic fibroblasts with no evidence of integration/nof the reprogramming vector in their genome, achieved by nucleofection/nof a polycistronic construct coexpressing Oct4, Sox2, Klf4,/nand c-Myc | |
| dc.format.mimetype | application/pdf | ca |
| dc.identifier.citation | González F, Barragan M, Tiscornia G, Montserrat N, Vassena R, Batlle L et al. Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector. Proc Natl Acad Sci U S A. 2009;106(22):8918-22. DOI: 10.1073/pnas.0901471106 | |
| dc.identifier.doi | http://dx.doi.org/10.1073/pnas.0901471106 | |
| dc.identifier.issn | 0027-8424 | |
| dc.identifier.uri | http://hdl.handle.net/10230/12427 | |
| dc.language.iso | eng | ca |
| dc.publisher | National Academy of Sciences | ca |
| dc.relation.ispartof | Proceedings of the National Academy of Sciences of the United States of America. 2009;106(22):8918-22 | |
| dc.rights | © National Academy of Sciences | ca |
| dc.rights.accessRights | info:eu-repo/semantics/openAccess | |
| dc.subject.other | Medicina regenerativa | |
| dc.subject.other | Cél·lules mare embrionàries | |
| dc.title | Generation of mouse-induced pluripotent stem cells by transient expression of a single nonviral polycistronic vector | ca |
| dc.type | info:eu-repo/semantics/article | ca |
| dc.type.version | info:eu-repo/semantics/publishedVersion |
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