Synthetically programmed antioxidant delivery by a domesticated skin commensal
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- dc.contributor.author Nevot, Guillermo
- dc.contributor.author Santos Moreno, Javier
- dc.contributor.author Campamà-Sanz, Nil
- dc.contributor.author Toloza, Lorena
- dc.contributor.author Parra-Cid, Cristóbal
- dc.contributor.author Jansen, Patrick A. M.
- dc.contributor.author Barbier, Içvara
- dc.contributor.author Ledesma-Amaro, Rodrigo
- dc.contributor.author van den Bogaard, Ellen H.
- dc.contributor.author Güell Cargol, Marc, 1982-
- dc.date.accessioned 2025-03-18T07:46:09Z
- dc.date.available 2025-03-18T07:46:09Z
- dc.date.issued 2025
- dc.description.abstract Bacteria represent a promising dynamic delivery system for the treatment of disease. In the skin, the relevant location of Cutibacterium acnes within the hair follicle makes this bacterium an attractive chassis for dermal biotechnological applications. Here, we provide a genetic toolbox for the engineering of this traditionally intractable bacterium, including basic gene expression tools, biocontainment strategies, markerless genetic engineering, and dynamic transcriptional regulation. As a proof of concept, we develop an antioxidant-secreting strain capable of reducing oxidative stress in a UV stress model.
- dc.description.sponsorship The authors thank master’s students Marta Fa, Patricia Tresguerres, Anastasiya Petrenko, Carmen Argilés Rodríguez, and Saptashwa Datta and bachelor’s students Lucía López Alcaide, Roger Torra Vaquero, and Zeynep Yeni for their technical assistance during their research internships. In addition, we would like to thank Cristina Fernandez for her support and technical assistance. We would also like to thank Dr. Holger Brüggemann and Dr. Rolf Lood for kindly providing the AD24 and 09-9 C. acnes strains, respectively. This project was funded by SKINDEV “Skin microbial devices” by the European Innovation Council (EIC Pathfinder) (grant agreement no. 101098826) and the Office of Naval Research (award N62909-18-1-2155). J.S.-M. was funded by a Marie Skłodowska-Curie Individual Fellowship (European Union’s Horizon 2020 Research and Innovation Programme under Marie Skłodowska-Curie grant agreement 882387), a Juan de la Cierva Incorporación Fellowship (grant no. IJC2020-043621-I funded by MICIU/AEI/10.13039/501100011033 and by European Union NextGenerationEU/PRTR), and a DCEXS MM postdoctoral project (Unidad de Excelencia María de Maeztu, funded by the AEI, ref. no. CEX2018-000792-M). G.N. was funded by an FI Fellowship (AGAUR-Catalan Government) co-funded by the European Social Fund (award 2020 FI-B 00107).
- dc.format.mimetype application/pdf
- dc.identifier.citation Nevot G, Santos-Moreno J, Campamà-Sanz N, Toloza L, Parra-Cid C, Jansen PAM, et al. Synthetically programmed antioxidant delivery by a domesticated skin commensal. Cell Syst. 2025 Feb 19;16(2):101169. DOI: 10.1016/j.cels.2025.101169
- dc.identifier.doi http://dx.doi.org/10.1016/j.cels.2025.101169
- dc.identifier.issn 2405-4720
- dc.identifier.uri http://hdl.handle.net/10230/69961
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Cell Syst. 2025 Feb 19;16(2):101169
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/882387
- dc.relation.projectID info:eu-repo/grantAgreement/EC/HE/101098826
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/IJC2020-043621-I
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/CEX2018-000792-M
- dc.rights © 2025 The Author(s). Published by Elsevier Inc. This is an open access article under the CC BY license (http://creativecommons.org/licenses/by/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.keyword CRISPRi
- dc.subject.keyword Cutibacterium acnes
- dc.subject.keyword SOD
- dc.subject.keyword Auxotrophies
- dc.subject.keyword Genetic toolkit
- dc.subject.keyword Keratinocytes
- dc.subject.keyword Microbiome engineering
- dc.subject.keyword Plasmid
- dc.subject.keyword Skin
- dc.subject.keyword Synthetic biology
- dc.title Synthetically programmed antioxidant delivery by a domesticated skin commensal
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion