Dietary consumption of Type 2 resistant starch and d-fagomine delays progression of metabolic disturbances in male rats on high-fat diet

Miralles-Pérez, Bernat; Ramos-Romero, Sara; Charpentier, María José; Sánchez-Martos, Vanessa; Fortuño-Mar, Àngels; Ponomarenko, Julia; Amézqueta, Susana; Piñol-Piñol, David; Zhang, Xiang; Torres, Josep Lluís; Romeu, Marta

doi:http://dx.doi.org/10.1002/mnfr.70230

Dietary consumption of Type 2 resistant starch and d-fagomine delays progression of metabolic disturbances in male rats on high-fat diet

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Miralles-Pérez B, Ramos-Romero S, Charpentier MJ, Sánchez-Martos V, Fortuño-Mar À, Ponomarenko J, et al. Dietary consumption of Type 2 resistant starch and d-fagomine delays progression of metabolic disturbances in male rats on high-fat diet. Mol Nutr Food Res. 2025 Sep 9:e70230. DOI: 10.1002/mnfr.70230

Abstract

High-fat (HF) diets contribute to obesity, insulin resistance, fatty liver, gut microbiota dysbiosis, oxidative stress, and low-grade chronic inflammation. This study evaluated the preventive effects of dietary Type 2 resistant starch (RS2) from high-amylose maize and low-dose d-fagomine (FG) from buckwheat on these metabolic disturbances. Male Wistar-Kyoto rats (9-10 weeks old) were assigned to four diet groups for 10 weeks: standard (STD) diet, HF diet (45% kcal from fat), HF + RS diet (15% RS2), and HF + FG diet (0.1% FG). Body characteristics, metabolic parameters, oxidative stress, gut microbiota, short-chain fatty acids (SCFAs), and eicosanoids were analyzed. Both HF + RS and HF + FG diets reduced perigonadal fat, plasma triacylglycerols, and oxidative stress. HF + RS diet improved glucose tolerance without significantly affecting insulin sensitivity, while HF + FG diet showed a tendency for improvement at later stages. Additionally, HF + RS diet showed greater beneficial effects on body weight and liver steatosis than HF + FG diet, likely due to gut microbiota and SCFA modulation. RS2 exerted stronger metabolic effects than FG under HF diet conditions, suggesting its greater potential in mitigating obesity-related complications. FG effects may require longer exposure to manifest.