The INO80 complex activates the transcription of S phase genes in a cell cycle regulated manner

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  • dc.contributor.author Knezevic, Ivaca
  • dc.contributor.author González Medina, Alberto, 1990-ca
  • dc.contributor.author Gaspa, Lauraca
  • dc.contributor.author Hidalgo Hernando, Elenaca
  • dc.contributor.author Ayté del Olmo, Joséca
  • dc.date.accessioned 2018-09-17T09:34:52Z
  • dc.date.issued 2018
  • dc.description.abstract Chromatin structure is an essential factor in the proper regulation of DNA repair, DNA replication and transcription. The INO80 complex and the SWR complex have been shown to play a fundamental role in transcription regulation through remodelling chromatin at specific genes and loci. Here we report that the Schizosaccharomyces pombe INO80 complex physically interacts with the MBF complex. Furthermore, we are able to detect the INO80 complex in MBF-regulated promoters. Binding of INO80 to these genes is cell-cycle regulated, with a maximum binding preceding their transcription and accumulation of their mRNAs. In fact, the INO80 complex is required to fully and timely activate the transcription of these genes. We also show that the accumulation of acetylated H2A.Z at the +1 nucleosome is cell-cycle regulated. Cells in which H2A.Z acetylation is abolished still have some cell cycle regulated transcription of MBF-dependent genes, although to a much lesser extent. This article is protected by copyright. All rights reserved.
  • dc.description.sponsorship This work was supported by grants from the Spanish Ministerio de Economia y Competitividad (BFU2015-66347-P), PLAN E and Feder, by MEIONet (BFU2015-71786-REDT) and by Unidad de Excelencia María de Maeztu (MDM-2014-0370). EH is recipient of an ICREA Academia Award (Generalitat de Catalunya)
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Knezevic I, González-Medina A, Gaspa L, Hidalgo E, Ayté J. The INO80 complex activates the transcription of S phase genes in a cell cycle regulated manner. FEBS J. 2018;285(20):3870-81. DOI: 10.1111/febs.14640
  • dc.identifier.doi http://dx.doi.org/10.1111/febs.14640
  • dc.identifier.issn 1742-4658
  • dc.identifier.uri http://hdl.handle.net/10230/35453
  • dc.language.iso eng
  • dc.publisher Wileyca
  • dc.relation.ispartof FEBS J. 2018;285(20):3870-81
  • dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-66347-P
  • dc.rights This is the peer reviewed version of the following article: Knezevic I, González-Medina A, Gaspa L, Hidalgo E, Ayté J. The INO80 complex activates the transcription of S phase genes in a cell cycle regulated manner. FEBS J. 2018 Aug 22. [Epub ahead of print], which has been published in final form at http://dx.doi.org/10.1111/febs.14640. This article may be used for non-commercial purposes in accordance with Wiley Terms and Conditions for Self-Archiving
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.subject.other Genètica
  • dc.subject.other Cromatina
  • dc.subject.other Schizosaccharomyces pombe
  • dc.title The INO80 complex activates the transcription of S phase genes in a cell cycle regulated mannerca
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/acceptedVersion

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