The INO80 complex activates the transcription of S phase genes in a cell cycle regulated manner

Abstract

Chromatin structure is an essential factor in the proper regulation of DNA repair, DNA replication and transcription. The INO80 complex and the SWR complex have been shown to play a fundamental role in transcription regulation through remodelling chromatin at specific genes and loci. Here we report that the Schizosaccharomyces pombe INO80 complex physically interacts with the MBF complex. Furthermore, we are able to detect the INO80 complex in MBF-regulated promoters. Binding of INO80 to these genes is cell-cycle regulated, with a maximum binding preceding their transcription and accumulation of their mRNAs. In fact, the INO80 complex is required to fully and timely activate the transcription of these genes. We also show that the accumulation of acetylated H2A.Z at the +1 nucleosome is cell-cycle regulated. Cells in which H2A.Z acetylation is abolished still have some cell cycle regulated transcription of MBF-dependent genes, although to a much lesser extent. This article is protected by copyright. All rights reserved.