Two-parameter single-molecule analysis for measurement of chromatin mobility

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  • dc.contributor.author Lerner, Jonathan
  • dc.contributor.author Gómez García, Pablo
  • dc.contributor.author McCarthy, Ryan L.
  • dc.contributor.author Liu, Zhe
  • dc.contributor.author Lakadamyali, Melike
  • dc.contributor.author Zaret, Kenneth S.
  • dc.date.accessioned 2022-06-14T09:05:33Z
  • dc.date.available 2022-06-14T09:05:33Z
  • dc.date.issued 2020
  • dc.description.abstract This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020).
  • dc.description.sponsorship We thank Luke Lavis for kindly providing the Halo ligand coupled to JF549 fluorophore. J.L. was supported by a fellowship from Fondation pour la Recherche Medicale, code 40334. M.L. was supported by a Linda Pechenik Montague Investigator Award. The research was supported by NIH grant R01GM36477 to K.S.Z.
  • dc.format.mimetype application/pdf
  • dc.identifier.citation Lerner J, Gómez-García PA, McCarthy RL, Liu Z, Lakadamyali M, Zaret KS. Two-parameter single-molecule analysis for measurement of chromatin mobility. STAR Protoc. 2020 Dec 13;1(3):100223. DOI:10.1016/j.xpro.2020.100223
  • dc.identifier.doi http://dx.doi.org/10.1016/j.xpro.2020.100223
  • dc.identifier.issn 2666-1667
  • dc.identifier.uri http://hdl.handle.net/10230/53489
  • dc.language.iso eng
  • dc.publisher Elsevier
  • dc.rights © Jonathan Lerner et al 2020. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
  • dc.rights.accessRights info:eu-repo/semantics/openAccess
  • dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
  • dc.subject.other Cromatina
  • dc.subject.other Proteïnes
  • dc.subject.other Molècules
  • dc.title Two-parameter single-molecule analysis for measurement of chromatin mobility
  • dc.type info:eu-repo/semantics/article
  • dc.type.version info:eu-repo/semantics/publishedVersion

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