Two-parameter single-molecule analysis for measurement of chromatin mobility

Lerner, Jonathan; Gómez García, Pablo; McCarthy, Ryan L.; Liu, Zhe; Lakadamyali, Melike; Zaret, Kenneth S.

doi:http://dx.doi.org/10.1016/j.xpro.2020.100223

Two-parameter single-molecule analysis for measurement of chromatin mobility

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Lerner J, Gómez-García PA, McCarthy RL, Liu Z, Lakadamyali M, Zaret KS. Two-parameter single-molecule analysis for measurement of chromatin mobility. STAR Protoc. 2020 Dec 13;1(3):100223. DOI:10.1016/j.xpro.2020.100223

Abstract

This protocol provides a two-parameter analysis of single-molecule tracking (SMT) trajectories of Halo-tagged histones in living adherent cell lines and unveils a chromatin mobility landscape composed of five chromatin types, ranging from low to high mobility. When the analysis is applied to Halo-tagged, chromatin-binding proteins, it associates chromatin interaction properties with known functions in a way that previously used SMT parameters did not. For complete information on the use and execution of this protocol, please refer to Lerner et al. (2020).