Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
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- dc.contributor.author Percy, Andrew J.
- dc.contributor.author Espadas, Guadalupe
- dc.contributor.author Sabidó Aguadé, Eduard, 1981-
- dc.contributor.author Borchers, Christoph H.
- dc.date.accessioned 2024-10-22T06:27:31Z
- dc.date.available 2024-10-22T06:27:31Z
- dc.date.issued 2015
- dc.description.abstract The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.
- dc.format.mimetype application/pdf
- dc.identifier.citation Percy AJ, Tamura-Wells J, Albar JP, Aloria K, Amirkhani A, Araujo GD, et al. Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment. EuPA Open Proteom. 2015 Sep;8:6-15. DOI: 10.1016/j.euprot.2015.06.001
- dc.identifier.doi http://dx.doi.org/10.1016/j.euprot.2015.06.001
- dc.identifier.issn 2212-9685
- dc.identifier.uri http://hdl.handle.net/10230/68293
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof EuPA Open Proteomics. 2015 Sep;8:6-15
- dc.rights © 2015 The Authors. Published by Elsevier GmbH. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/).
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.keyword Accuracy
- dc.subject.keyword Human plasma
- dc.subject.keyword Quality control
- dc.subject.keyword Quantitative proteomics
- dc.subject.keyword Reproducibility
- dc.subject.keyword Workflow validation
- dc.title Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion