Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment

Percy, Andrew J.; Espadas, Guadalupe; Sabidó Aguadé, Eduard, 1981-; Borchers, Christoph H.

doi:http://dx.doi.org/10.1016/j.euprot.2015.06.001

Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment

Llicència

Citació

Percy AJ, Tamura-Wells J, Albar JP, Aloria K, Amirkhani A, Araujo GD, et al. Inter-laboratory evaluation of instrument platforms and experimental workflows for quantitative accuracy and reproducibility assessment. EuPA Open Proteom. 2015 Sep;8:6-15. DOI: 10.1016/j.euprot.2015.06.001

Resum

The reproducibility of plasma protein quantitation between laboratories and between instrument types was examined in a large-scale international study involving 16 laboratories and 19 LC–MS/MS platforms, using two kits designed to evaluate instrument performance and one kit designed to evaluate the entire bottom-up workflow. There was little effect of instrument type on the quality of the results, demonstrating the robustness of LC/MRM-MS with isotopically labeled standards. Technician skill was a factor, as errors in sample preparation and sub-optimal LC–MS performance were evident. This highlights the importance of proper training and routine quality control before quantitation is done on patient samples.