Detailed quantification of cardiac ventricular myocardial architecture in the embryonic and fetal mouse heart by application of structure tensor analysis to high resolution episcopic microscopic data

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• dc.contributor.author García Cañadilla, Patricia, 1985-
• dc.contributor.author Mohun, Timothy J.
• dc.contributor.author Bijnens, Bart
• dc.contributor.author Cook, Andrew C.
• dc.date.accessioned 2025-10-20T06:19:26Z
• dc.date.available 2025-10-20T06:19:26Z
• dc.date.issued 2022
• dc.description.abstract The mammalian heart, which is one of the first organs to form and function during embryogenesis, develops from a simple tube into a complex organ able to efficiently pump blood towards the rest of the body. The progressive growth of the compact myocardium during embryonic development is accompanied by changes in its structural complexity and organisation. However, how myocardial myoarchitecture develops during embryogenesis remain poorly understood. To date, analysis of heart development has focused mainly on qualitative descriptions using selected 2D histological sections. High resolution episcopic microscopy (HREM) is a novel microscopic imaging technique that enables to obtain high-resolution three-dimensional images of the heart and perform detailed quantitative analyses of heart development. In this work, we performed a detailed characterization of the development of myocardial architecture in wildtype mice, from E14.5 to E18.5, by means of structure tensor analysis applied to HREM images of the heart. Our results shows that even at E14.5, myocytes are already aligned, showing a gradual change in their helical angle from positive angulation in the endocardium towards negative angulation in the epicardium. Moreover, there is gradual increase in the degree of myocardial organisation concomitant with myocardial growth. However, the development of the myoarchitecture is heterogeneous showing regional differences between ventricles, ventricular walls as well as between myocardial layers, with different growth patterning between the endocardium and epicardium. We also found that the percentage of circumferentially arranged myocytes within the LV significantly increases with gestational age. Finally, we found that fractional anisotropy (FA) within the LV gradually increases with gestational age, while the FA within RV remains unchanged.en
• dc.description.sponsorship PG-C has received funding from the postdoctoral fellowships program Beatriu de Pinos (2018-BP-00201), funded by the Secretary of Universities and Research (Goverment of Catalonia) and by the Horizon 2020 programme of research and innovation of the European Union under the Marie Skłodowska-Curie grant agreement N° 801370. This research was funded in whole, or in part, by the funded by the Wellcome Trust (100160) and the Francis Crick Institute, which receives its core funding from Cancer Research UK (FC001117), the UK Medical Research Council (FC001117), and the Wellcome Trust (FC001117). For the purpose of Open Access, the author has applied a CC BY public copyright licence to any Author Accepted Manuscript version arising from this submission.en
• dc.format.mimetype application/pdf
• dc.identifier.citation Garcia-Canadilla P, Mohun TJ, Bijnens B, Cook AC. Detailed quantification of cardiac ventricular myocardial architecture in the embryonic and fetal mouse heart by application of structure tensor analysis to high resolution episcopic microscopic data. Front Cell Dev Biol. 2022 Nov 16;10:1000684. DOI: 10.3389/fcell.2022.1000684
• dc.identifier.doi http://dx.doi.org/10.3389/fcell.2022.1000684
• dc.identifier.issn 2296-634X
• dc.identifier.uri http://hdl.handle.net/10230/71555
• dc.language.iso eng
• dc.publisher Frontiers
• dc.relation.ispartof Frontiers in Cell and Developmental Biology. 2022 Nov 16;10:1000684
• dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/801370
• dc.rights © 2022 Garcia-Canadilla, Mohun, Bijnens and Cook. This is an open-access article distributed under the terms of the Creative Commons Attribution License (CC BY). The use, distribution or reproduction in other forums is permitted, provided the original author(s) and the copyright owner(s) are credited and that the original publication in this journal is cited, in accordance with accepted academic practice. No use, distribution or reproduction is permitted which does not comply with these terms.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Myocyte orientationen
• dc.subject.keyword Myocardial developmenten
• dc.subject.keyword High resolution episcopic microscopyen
• dc.subject.keyword Structure tensor analysisen
• dc.subject.keyword Helical angle (HA)en
• dc.subject.keyword Intrusion angleen
• dc.title Detailed quantification of cardiac ventricular myocardial architecture in the embryonic and fetal mouse heart by application of structure tensor analysis to high resolution episcopic microscopic dataen
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion