A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells
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- dc.contributor.author Martin, Laura
- dc.contributor.author Vicario, Chiara
- dc.contributor.author Castells García, Àlvaro, 1991-
- dc.contributor.author Lakadamyali, Melike
- dc.contributor.author Neguembor, Maria Victoria
- dc.contributor.author Cosma, Maria Pia
- dc.date.accessioned 2022-01-31T12:23:36Z
- dc.date.available 2022-01-31T12:23:36Z
- dc.date.issued 2021
- dc.description.abstract Here, we describe three complementary microscopy-based approaches to quantify morphological changes of chromatin organization in cultured adherent cells: the analysis of the coefficient of variation of DNA, the measurement of DNA-free nuclear areas, and the quantification of chromatin-associated proteins at the nuclear edge. These approaches rely on confocal imaging and stochastic optical reconstruction microscopy and allow a fast and robust quantification of chromatin compaction. These approaches circumvent inter-variability between imaging conditions and apply to every type of adherent cells. For complete details on the use and execution of this protocol, please refer to Neguembor et al. (2021).
- dc.description.sponsorship The authors acknowledge the support from European Union's Horizon 2020 Research and Innovation Programme (CellViewer no. 686637 to M.P.C. and M.L.); Ministerio de Ciencia e Innovación, grant (BFU2017-86760-P [AEI/FEDER, UE] to M.P.C.), and an AGAUR grant from Secretaria d’Universitats i Recerca del Departament d’Empresa i Coneixement de la Generalitat de Catalunya ([2017 SGR 689] to M.P.C.); Centro de Excelencia Severo Ochoa (2013–2017 to M.P.C.); CERCA Programme/Generalitat de Catalunya (to M.P.C.); the Spanish Ministry of Science and Innovation to the EMBL partnership (to M.P.C.); National Natural Science Foundation of China (31971177 to M.P.C.); Innovative Team Program of Guangzhou Regenerative Medicine and Health Guangdong Laboratory (2018GZR110103001 to M.P.C.); People Program (Marie Curie Actions) FP7/2007–2013 under REA grant 608959 (to M.V.N.); Juan de la Cierva-Incorporación 2017 (to M.V.N.); grant for the recruitment of early-stage research staff FI-2020 (Operational Program of Catalonia 2014-2020 CCI 2014ES05SFOP007 of the European Social Fund to L.M.); "La Caixa" Foundation Fellowship (ID 100010434, #LCF/BQ/DR20/11790016) (to L.M.); and "La Caixa-Severo Ochoa" pre-doctoral fellowship (to A.C.-G.)
- dc.format.mimetype application/pdf
- dc.identifier.citation Martin L, Vicario C, Castells-García A, Lakadamyali M, Neguembor MV, Cosma MP. A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells. STAR Protoc. 2021 Sep 30; 2(4): 100865. DOI: 10.1016/j.xpro.2021.100865
- dc.identifier.doi http://dx.doi.org/10.1016/j.xpro.2021.100865
- dc.identifier.issn 2666-1667
- dc.identifier.uri http://hdl.handle.net/10230/52374
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/686637
- dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/608959
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BFU2017-86760-P
- dc.rights © 2021 Laura Martin et al. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.other Citologia
- dc.subject.other Microscòpia
- dc.subject.other Biologia molecular
- dc.title A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion