A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells
A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells
Citació
- Martin L, Vicario C, Castells-García A, Lakadamyali M, Neguembor MV, Cosma MP. A protocol to quantify chromatin compaction with confocal and super-resolution microscopy in cultured cells. STAR Protoc. 2021 Sep 30; 2(4): 100865. DOI: 10.1016/j.xpro.2021.100865
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Descripció
Resum
Here, we describe three complementary microscopy-based approaches to quantify morphological changes of chromatin organization in cultured adherent cells: the analysis of the coefficient of variation of DNA, the measurement of DNA-free nuclear areas, and the quantification of chromatin-associated proteins at the nuclear edge. These approaches rely on confocal imaging and stochastic optical reconstruction microscopy and allow a fast and robust quantification of chromatin compaction. These approaches circumvent inter-variability between imaging conditions and apply to every type of adherent cells. For complete details on the use and execution of this protocol, please refer to Neguembor et al. (2021).