DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis

Rosas Salvans, Miquel, 1987-; Scrofani, Jacopo, 1984-; Modol, Aitor; Vernos, Isabelle, 1959-

doi:http://dx.doi.org/10.1242/jcs.227033

DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis

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Rosas-Salvans M, Scrofani J, Modol A, Vernos I. DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis. J Cell Sci. 2019 Jun 3; 132(11). DOI 10.1242/jcs.227033

Abstract

Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions, increasing its processivity and force production. Here, we show that DnaJB6 is a novel RanGTP-regulated protein. It interacts with the dynactin subunit p150Glued (also known as DCTN1) in a RanGTP-dependent manner specifically in M-phase, and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase.