DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis

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• dc.contributor.author Rosas Salvans, Miquel, 1987-
• dc.contributor.author Scrofani, Jacopo, 1984-
• dc.contributor.author Modol, Aitor
• dc.contributor.author Vernos, Isabelle, 1959-
• dc.date.accessioned 2019-09-20T10:53:31Z
• dc.date.available 2019-09-20T10:53:31Z
• dc.date.issued 2019
• dc.description.abstract Bipolar spindle organization is essential for the faithful segregation of chromosomes during cell division. This organization relies on the collective activities of motor proteins. The minus-end-directed dynein motor complex generates spindle inward forces and plays a major role in spindle pole focusing. The dynactin complex regulates many dynein functions, increasing its processivity and force production. Here, we show that DnaJB6 is a novel RanGTP-regulated protein. It interacts with the dynactin subunit p150Glued (also known as DCTN1) in a RanGTP-dependent manner specifically in M-phase, and promotes spindle pole focusing and dynein force generation. Our data suggest a novel mechanism by which RanGTP regulates dynein activity during M-phase.
• dc.description.sponsorship We acknowledge the Spanish Ministry of Economy, Industry and Competitiveness (Ministerio de Economía y Competitividad) to the EMBL partnership and support of the Spanish Ministry of Economy and Competitiveness, ‘Centro de Excelencia Severo Ochoa’ as well as support of the CERCA Programme/Generalitat de Catalunya. M.R. was supported by a FPI fellowship from the Spanish Ministry of Economy BES-2013-064601. Work in the Vernos laboratory was supported by grants from the Spanish Ministry of Economy and Competitiveness I+D (grants BFU2012-37163 and BFU2015-68726-P). Deposited in PMC for immediate release.
• dc.format.mimetype application/pdf
• dc.identifier.citation Rosas-Salvans M, Scrofani J, Modol A, Vernos I. DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis. J Cell Sci. 2019 Jun 3; 132(11). DOI 10.1242/jcs.227033
• dc.identifier.doi http://dx.doi.org/10.1242/jcs.227033
• dc.identifier.issn 0021-9533
• dc.identifier.uri http://hdl.handle.net/10230/42307
• dc.language.iso eng
• dc.publisher Company of Biologists
• dc.relation.ispartof J Cell Sci. 2019 Jun 3; 132(11)
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BES-2013-064601
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-37163
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/BFU2015-68726-P
• dc.rights © Company of Biologists. http://dx.doi.org/10.1242/jcs.227033. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution and reproduction in any medium provided that the original work is properly attributed
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0
• dc.subject.keyword DnaJB6
• dc.subject.keyword Dynein
• dc.subject.keyword Microtubules
• dc.subject.keyword RanGTP
• dc.subject.keyword Spindle organization
• dc.title DnaJB6 is a RanGTP-regulated protein required for microtubule organization during mitosis
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion