The Hog1 stress-activated protein kinase targets nucleoporins to control mRNA export upon stress

Regot Rodríguez de Mier, Sergi; Nadal Clanchet, Eulàlia de; Rodríguez-Navarro, Susana; Seisenbacher, Gerhard; González Novo, Alberto; Pérez-Fernandez, Jorge; Gadal, Olivier; Ammerer, Gustav; Posas Garriga, Francesc

doi:http://dx.doi.org/10.1074/jbc.M112.444042

The Hog1 stress-activated protein kinase targets nucleoporins to control mRNA export upon stress

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Regot S, de Nadal E, Rodríguez-Navarro S, González-Novo A, Pérez-Fernandez J, Gadal O, et al. The Hog1 stress-activated protein kinase targets nucleoporins to control mRNA export upon stress. J Biol Chem. 2013 Jun;288(24):17384-98. DOI: 10.1074/jbc.M112.444042

Abstract

The control of mRNA biogenesis is exerted at several steps. In response to extracellular stimuli, stress-activated protein kinases (SAPK) modulate gene expression to maximize cell survival. In yeast, the Hog1 SAPK plays a key role in reprogramming the gene expression pattern required for cell survival upon osmostress by acting during transcriptional initiation and elongation. Here, we genetically show that an intact nuclear pore complex is important for cell survival and maximal expression of stress-responsive genes. The Hog1 SAPK associates with nuclear pore complex components and directly phosphorylates the Nup1, Nup2, and Nup60 components of the inner nuclear basket. Mutation of those factors resulted in a deficient export of stress-responsive genes upon stress. Association of Nup1, Nup2, and Nup60 to stress-responsive promoters occurs upon stress depending on Hog1 activity. Accordingly, STL1 gene territory is maintained at the nuclear periphery upon osmostress in a Hog1-dependent manner. Cells containing non-phosphorylatable mutants in Nup1 or Nup2 display reduced expression of stress-responsive genes. Together, proper mRNA biogenesis of stress-responsive genes requires of the coordinate action of synthesis and export machineries by the Hog1 SAPK. Background: The Hog1 stress-activated protein kinase (SAPK) regulates mRNA biogenesis in response to osmostress. Results: The nuclear pore complex is regulated upon osmostress by the Hog1 SAPK. Conclusion: mRNA export is controlled by the HOG signaling pathway. Significance: Efficient mRNA biogenesis of stress-responsive genes requires of the coordination between synthesis and mRNA export.