The Hog1 stress-activated protein kinase targets nucleoporins to control mRNA export upon stress

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• dc.contributor.author Regot Rodríguez de Mier, Sergi
• dc.contributor.author Nadal Clanchet, Eulàlia de
• dc.contributor.author Rodríguez-Navarro, Susana
• dc.contributor.author Seisenbacher, Gerhard
• dc.contributor.author González Novo, Alberto
• dc.contributor.author Pérez-Fernandez, Jorge
• dc.contributor.author Gadal, Olivier
• dc.contributor.author Ammerer, Gustav
• dc.contributor.author Posas Garriga, Francesc
• dc.date.accessioned 2024-12-03T07:33:40Z
• dc.date.available 2024-12-03T07:33:40Z
• dc.date.issued 2013
• dc.description.abstract The control of mRNA biogenesis is exerted at several steps. In response to extracellular stimuli, stress-activated protein kinases (SAPK) modulate gene expression to maximize cell survival. In yeast, the Hog1 SAPK plays a key role in reprogramming the gene expression pattern required for cell survival upon osmostress by acting during transcriptional initiation and elongation. Here, we genetically show that an intact nuclear pore complex is important for cell survival and maximal expression of stress-responsive genes. The Hog1 SAPK associates with nuclear pore complex components and directly phosphorylates the Nup1, Nup2, and Nup60 components of the inner nuclear basket. Mutation of those factors resulted in a deficient export of stress-responsive genes upon stress. Association of Nup1, Nup2, and Nup60 to stress-responsive promoters occurs upon stress depending on Hog1 activity. Accordingly, STL1 gene territory is maintained at the nuclear periphery upon osmostress in a Hog1-dependent manner. Cells containing non-phosphorylatable mutants in Nup1 or Nup2 display reduced expression of stress-responsive genes. Together, proper mRNA biogenesis of stress-responsive genes requires of the coordinate action of synthesis and export machineries by the Hog1 SAPK. Background: The Hog1 stress-activated protein kinase (SAPK) regulates mRNA biogenesis in response to osmostress. Results: The nuclear pore complex is regulated upon osmostress by the Hog1 SAPK. Conclusion: mRNA export is controlled by the HOG signaling pathway. Significance: Efficient mRNA biogenesis of stress-responsive genes requires of the coordination between synthesis and mRNA export.
• dc.description.sponsorship This work was supported by MINECO (Spanish government) Grant BFU2012-33503, the Consolider Ingenio 2010 program (Grant CSD2007-0015), the Fundación Marcelino Botín (to F.P.), and Grant BFU2011-26722 (to E.d.N.).
• dc.format.mimetype application/pdf
• dc.identifier.citation Regot S, de Nadal E, Rodríguez-Navarro S, González-Novo A, Pérez-Fernandez J, Gadal O, et al. The Hog1 stress-activated protein kinase targets nucleoporins to control mRNA export upon stress. J Biol Chem. 2013 Jun;288(24):17384-98. DOI: 10.1074/jbc.M112.444042
• dc.identifier.doi http://dx.doi.org/10.1074/jbc.M112.444042
• dc.identifier.issn 0021-9258
• dc.identifier.uri http://hdl.handle.net/10230/68893
• dc.language.iso eng
• dc.publisher American Society for Biochemistry and Molecular Biology (ASBMB)
• dc.relation.ispartof The Journal of Biological Chemistry. 2013 Jun;288(24):17384-98
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PN/CSD2007-0015
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2012-33503
• dc.relation.projectID info:eu-repo/grantAgreement/ES/3PN/BFU2011-26722
• dc.rights © 2013 ASBMB. Currently published by Elsevier Inc; originally published by American Society for Biochemistry and Molecular Biology. This is an open access article distributed under the terms of the Creative Commons CC-BY license, which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly cited.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Gene expression
• dc.subject.keyword MAP Kinases (MAPKs)
• dc.subject.keyword p38 MAPK
• dc.subject.keyword Signal transduction
• dc.subject.keyword Stress response
• dc.subject.keyword mRNA export
• dc.subject.keyword Yeast
• dc.title The Hog1 stress-activated protein kinase targets nucleoporins to control mRNA export upon stress
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion