Use of linear ion traps in data-independent acquisition methods benefits low-input proteomics
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- dc.contributor.author Borràs, Eva
- dc.contributor.author Pastor, Olga
- dc.contributor.author Sabidó Aguadé, Eduard, 1981-
- dc.date.accessioned 2021-12-21T11:07:13Z
- dc.date.available 2021-12-21T11:07:13Z
- dc.date.issued 2021
- dc.description.abstract The need for a better understanding of cellular heterogeneity has pushed mass spectrometry technologies to the analysis of single-cell and single-cell-type proteomes, although several challenges still limit their widespread implementation. Among the efforts toward single-cell and low-input analyses, there is the adoption of data-independent acquisition methods to increase analytical sensitivity. Here, we revisited the use of linear ion traps mass analyzers in data-independent acquisition methods and demonstrate their benefits to boost peptide and protein identifications in low-input proteomes.en
- dc.description.sponsorship Funding: the CRG/UPF Proteomics Unit is part of the Spanish Infrastructure for Omics Technologies (ICTS OmicsTech) and it is member of ProteoRed PRB3 consortium which is supported by Grant PT17/0019 of the PE I+D+i 2013-2016 from the Instituto de Salud Carlos III (ISCIII), ERDF, and “Secretaria d’Universitats i Recerca del Departament d’Economia i Coneixement de la Generalitat de Catalunya” (2017SGR595). This project was supported by EPIC-XS, Project Number 823839, funded by the Horizon 2020 programme of the European Union. We also acknowledge support of the Spanish Ministry of Science and Innovation to the EMBL partnership, the Centro de Excelencia Severo Ochoa, and the CERCA Programme/Generalitat de Catalunyaen
- dc.format.mimetype application/pdf
- dc.identifier.citation Borràs E, Pastor O, Sabidó E. Use of linear ion traps in data-independent acquisition methods benefits low-input proteomics. Anal Chem. 2021 Aug 31;93(34):11649-11653. DOI: 10.1021/acs.analchem.1c01885
- dc.identifier.doi http://dx.doi.org/10.1021/acs.analchem.1c01885
- dc.identifier.issn 1520-6882
- dc.identifier.uri http://hdl.handle.net/10230/51906
- dc.language.iso eng
- dc.publisher American Chemical Society (ACS)
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/823839
- dc.rights © 2021 Eva Borràs, Olga Pastor i Eduard Sabidó. Published by American Chemical Society. https://creativecommons.org/licenses/by/4.0/
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by/4.0/
- dc.subject.other Genèticaca
- dc.subject.other Genòmicaca
- dc.subject.other Espectrometria de massesca
- dc.title Use of linear ion traps in data-independent acquisition methods benefits low-input proteomicsen
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion