Use of linear ion traps in data-independent acquisition methods benefits low-input proteomics

Borràs, Eva; Pastor, Olga; Sabidó Aguadé, Eduard, 1981-

doi:http://dx.doi.org/10.1021/acs.analchem.1c01885

Use of linear ion traps in data-independent acquisition methods benefits low-input proteomics

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Citació

Borràs E, Pastor O, Sabidó E. Use of linear ion traps in data-independent acquisition methods benefits low-input proteomics. Anal Chem. 2021 Aug 31;93(34):11649-11653. DOI: 10.1021/acs.analchem.1c01885

Resum

The need for a better understanding of cellular heterogeneity has pushed mass spectrometry technologies to the analysis of single-cell and single-cell-type proteomes, although several challenges still limit their widespread implementation. Among the efforts toward single-cell and low-input analyses, there is the adoption of data-independent acquisition methods to increase analytical sensitivity. Here, we revisited the use of linear ion traps mass analyzers in data-independent acquisition methods and demonstrate their benefits to boost peptide and protein identifications in low-input proteomes.