Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in rat model: Preliminary results on differential conductivity

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• dc.contributor.author Pañella Vilamú, Clara, 1987-
• dc.contributor.author Castellví Fernández, Quim
• dc.contributor.author Moll, Xavier
• dc.contributor.author Quesada Diez, Rita
• dc.contributor.author Villanueva, Alberto
• dc.contributor.author Iglesias, Mar
• dc.contributor.author Naranjo‐Hans, Dolores
• dc.contributor.author Sánchez Velázquez, Patricia, 1985-
• dc.contributor.author Andaluz, Anna
• dc.contributor.author Grande Posa, Luís
• dc.contributor.author Ivorra Cano, Antoni, 1974-
• dc.contributor.author Burdío Pinilla, Fernando
• dc.date.accessioned 2021-06-10T07:16:32Z
• dc.date.available 2021-06-10T07:16:32Z
• dc.date.issued 2017
• dc.description.abstract Background. Spread hepatic tumours are not suitable for treatment either by surgery or conventional ablation methods. The aim of this study was to evaluate feasibility and safety of selectively increasing the healthy hepatic conductivity by the hypersaline infusion (HI) through the portal vein. We hypothesize this will allow simultaneous safe treatment of all nodules by irreversible electroporation (IRE) when applied in a transhepatic fashion. Material and methods. Sprague Dawley (Group A, n = 10) and Athymic rats with implanted hepatic tumour (Group B, n = 8) were employed. HI was performed (NaCl 20%, 3.8 mL/Kg) by trans-splenic puncture. Deionized serum (40 mL/Kg) and furosemide (2 mL/Kg) were simultaneously infused through the jugular vein to compensate hypernatremia. Changes in conductivity were monitored in the hepatic and tumour tissue. The period in which hepatic conductivity was higher than tumour conductivity was defined as the therapeutic window (TW). Animals were monitored during 1-month follow-up. The animals were sacrificed and selective samples were used for histological analysis. Results. The overall survival rate was 82.4% after the HI protocol. The mean maximum hepatic conductivity after HI was 2.7 and 3.5 times higher than the baseline value, in group A and B, respectively. The mean maximum hepatic conductivity after HI was 1.4 times higher than tumour tissue in group B creating a TW to implement selective IRE. Conclusions. HI through the portal vein is safe when the hypersaline overload is compensated with deionized serum and it may provide a TW for focused IRE treatment on tumour nodules.
• dc.description.sponsorship This work received financial support from the Spanish Government (Ministry of Economy and Competitiveness) under Grant TEC2014-52383-C3. The authors disclose no conflicts of interests.
• dc.format.mimetype application/pdf
• dc.identifier.citation Pañella C, Castellví Q, Moll X, Quesada R, Villanueva A, Iglesias M, Naranjo D, Sánchez-Velázquez P, Andaluz A, Grande L, Ivorra A, Burdío F. Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in rat model: Preliminary results on differential conductivity. Radiol Oncol. 2017;51(4):415-21. DOI: 10.1515/raon-2017-0051
• dc.identifier.doi http://dx.doi.org/10.1515/raon-2017-0051
• dc.identifier.issn 1318-2099
• dc.identifier.uri http://hdl.handle.net/10230/47837
• dc.language.iso eng
• dc.publisher De Gruyter
• dc.relation.ispartof Radiology and Oncology. 2017;51(4):415-21
• dc.relation.projectID info:eu-repo/grantAgreement/ES/1PE/TEC2014-52383-C3
• dc.rights © De Gruyter Published version available at http://dx.doi.org/10.1515/raon-2017-0051 https://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri https://creativecommons.org/licenses/by-nc-nd/4.0/
• dc.subject.keyword Irreversible electroporation
• dc.subject.keyword Liver tumour
• dc.subject.keyword Electrical conductivity
• dc.title Focused transhepatic electroporation mediated by hypersaline infusion through the portal vein in rat model: Preliminary results on differential conductivity
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion