High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
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- dc.contributor.author Levkov, Klimentiy
- dc.contributor.author Linzon, Yoav
- dc.contributor.author Mercadal, Borja
- dc.contributor.author Ivorra Cano, Antoni, 1974-
- dc.contributor.author González, César Antonio
- dc.contributor.author Golberg, Alexander
- dc.date.accessioned 2020-03-12T15:41:19Z
- dc.date.issued 2020
- dc.description.abstract Optimization of protocols is required for each specific type of biomass processed by electroporation of the cell membrane with high voltage pulsed electric fields (PEF). Such optimization requires convenient and adaptable laboratory systems, which will enable determination of both electrical and mechanical parameters for successful electroporation and fractionation. In this work, we report on a laboratory PEF system consisting of a high voltage generator with a novel asymmetric voltage multiplying architecture and a treatment chamber with sliding electrodes. The system allows applying pulses of up to 4 kV and 1 kA with a pulse duration between 1 μs and 100 μs. The allowable energy dissipated per pulse on electroporated biomass is determined by the conditions for cooling the biomass in the electroporation cell. The device was tested on highly conductive green macroalgae from Ulva sp., a promising but challenging feedstock for the biorefinery. Successful electroporation was confirmed with bioimpedance measurements.en
- dc.format.mimetype application/pdf
- dc.identifier.citation Levkov K, Linzon Y, Mercadal B, Ivorra A, González CA, Golberg A. High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation. Innov Food Sci Emerg Technol. 2020 Mar;60:102288. DOI: 10.1016/j.ifset.2020.102288
- dc.identifier.doi http://dx.doi.org/10.1016/j.ifset.2020.102288
- dc.identifier.issn 1466-8564
- dc.identifier.uri http://hdl.handle.net/10230/43887
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Innovative Food Science & Emerging Technologies. 2020 Mar;60:102288
- dc.rights © Elsevier http://dx.doi.org/10.1016/j.ifset.2020.102288
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword Pulsed electric field generatoren
- dc.subject.keyword Electroporationen
- dc.subject.keyword Biomass processingen
- dc.subject.keyword Macroalgaeen
- dc.subject.keyword Bioimpedanceen
- dc.subject.keyword Bioeconomyen
- dc.title High-voltage pulsed electric field laboratory device with asymmetric voltage multiplier for marine macroalgae electroporation
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion