In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites

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• dc.contributor.author Grey, Corinneca
• dc.contributor.author Clément, Julie A.J.ca
• dc.contributor.author Buard, Jeromeca
• dc.contributor.author Leblanc, Benjaminca
• dc.contributor.author Gut, Ivo Glynneca
• dc.contributor.author Gut, Martaca
• dc.contributor.author Duret, Laurentca
• dc.contributor.author de Massy, Bernard D.ca
• dc.date.accessioned 2018-06-14T07:43:10Z
• dc.date.available 2018-06-14T07:43:10Z
• dc.date.issued 2017
• dc.description.abstract In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensive analysis of PRDM9 binding in mouse spermatocytes. Unexpectedly, we identified a noncanonical recruitment of PRDM9 to sites that lack recombination activity and the PRDM9 binding consensus motif. These sites include gene promoters, where PRDM9 is recruited in a DSB-dependent manner. Another subset reveals DSB-independent interactions between PRDM9 and genomic sites, such as the binding sites for the insulator protein CTCF. We propose that these DSB-independent sites result from interactions between hotspot-bound PRDM9 and genomic sequences located on the chromosome axis.
• dc.description.sponsorship The work leading to the H3K4me3 results received funding from the European Union Seventh Framework Programme (FP7/2007-2013) under grant agreement no. 262055. This work was supported by the Agence Nationale de la Recherche (ANR-15-CE12-0010-01/DaSiRe). B.d.M. was funded by grants from the Centre National pour la Recherche Scientifique (CNRS) and the European Research Council Executive Agency under the European Community's Seventh Framework Programme (FP7/2007-2013 grant agreement no. [322788]).
• dc.format.mimetype application/pdf
• dc.identifier.citation Grey C, Clément JA, Buard J, Leblanc B, Gut I, Gut M et al. In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites. Genome Res. 2017 Apr;27(4):580-90. DOI: 10.1101/gr.217240.116
• dc.identifier.doi http://dx.doi.org/10.1101/gr.217240.116
• dc.identifier.issn 1088-9051
• dc.identifier.uri http://hdl.handle.net/10230/34894
• dc.language.iso eng
• dc.publisher BioMed Centralca
• dc.relation.ispartof Genome Research. 2017 Apr;27(4):580-90
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/262055
• dc.relation.projectID info:eu-repo/grantAgreement/EC/FP7/322788
• dc.rights © 2017 Grey et al.; Published by Cold Spring Harbor Laboratory Press. This article, published in Genome Research, is available under a Creative Commons License (Attribution 4.0 International), as described at http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Protein binding
• dc.subject.keyword Prdm9 binding
• dc.subject.keyword Dna double-strand breaks
• dc.title In vivo binding of PRDM9 reveals interactions with noncanonical genomic sitesca
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion