In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites
In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites
Citació
- Grey C, Clément JA, Buard J, Leblanc B, Gut I, Gut M et al. In vivo binding of PRDM9 reveals interactions with noncanonical genomic sites. Genome Res. 2017 Apr;27(4):580-90. DOI: 10.1101/gr.217240.116
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Descripció
Resum
In mouse and human meiosis, DNA double-strand breaks (DSBs) initiate homologous recombination and occur at specific sites called hotspots. The localization of these sites is determined by the sequence-specific DNA binding domain of the PRDM9 histone methyl transferase. Here, we performed an extensive analysis of PRDM9 binding in mouse spermatocytes. Unexpectedly, we identified a noncanonical recruitment of PRDM9 to sites that lack recombination activity and the PRDM9 binding consensus motif. These sites include gene promoters, where PRDM9 is recruited in a DSB-dependent manner. Another subset reveals DSB-independent interactions between PRDM9 and genomic sites, such as the binding sites for the insulator protein CTCF. We propose that these DSB-independent sites result from interactions between hotspot-bound PRDM9 and genomic sequences located on the chromosome axis.