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Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq

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dc.contributor.author Valdés-Hernández, Jesús
dc.contributor.author Ramayo-Caldas, Yuliaxis
dc.contributor.author Passols, Magí
dc.contributor.author Sebastià, Cristina
dc.contributor.author Criado-Mesas, Lourdes
dc.contributor.author Crespo-Piazuelo, Daniel
dc.contributor.author Esteve-Codina, Anna
dc.contributor.author Castelló, Anna
dc.contributor.author Sánchez, Armand
dc.contributor.author Folch, Josep M.
dc.date.accessioned 2023-03-09T07:22:17Z
dc.date.available 2023-03-09T07:22:17Z
dc.date.issued 2023
dc.identifier.citation Valdés-Hernández J, Ramayo-Caldas Y, Passols M, Sebastià C, Criado-Mesas L, Crespo-Piazuelo D, Esteve-Codina A, Castelló A, Sánchez A, Folch JM. Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq. Sci Rep. 2023 Jan 11;13(1):535. DOI: 10.1038/s41598-022-27016-x
dc.identifier.issn 2045-2322
dc.identifier.uri http://hdl.handle.net/10230/56117
dc.description.abstract Fatty acids (FAs) play an essential role as mediators of cell signaling and signal transduction, affecting metabolic homeostasis and determining meat quality in pigs. However, FAs are transformed by the action of several genes, such as those encoding desaturases and elongases of FAs in lipogenic tissues. The aim of the current work was to identify candidate genes, biological processes, and pathways involved in the modulation of intramuscular FA profile from longissimus dorsi muscle. FA profile by gas chromatography of methyl esters and gene expression by RNA-Seq were determined in 129 Iberian × Duroc backcrossed pigs. An association analysis between the muscle transcriptome and its FA profile was performed, followed by a concordance and functional analysis. Overall, a list of well-known (e.g., PLIN1, LEP, ELOVL6, SC5D, NCOA2, ACSL1, MDH1, LPL, LGALS12, TFRC, GOT1, and FBP1) and novel (e.g., TRARG1, TANK, ENSSSCG00000011196, and ENSSSCG00000038429) candidate genes was identified, either in association with specific or several FA traits. Likewise, several of these genes belong to biological processes and pathways linked to energy, lipid, and carbohydrate metabolism, which seem determinants in the modulation of FA compositions. This study can contribute to elucidate the complex relationship between gene expression and FA profile in pig muscle.
dc.description.sponsorship This work was supported by the Spanish Ministerio de Ciencia e Innovación (MICINN) and the Fondo Europeo de Desarrollo Regional (FEDER) with project references: AGL2017-82641-R and PID2020-112677RB-C22. Jesús Valdés Hernández was funded with an FI PhD grant “Formació i contractació de personal investigador novell” fellowship provided by the “Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR)” from Generalitat de Catalunya (2019FI_B_00787 credential). Yuliaxis Ramayo-Caldas was funded by a “Ramon y Cajal” contract (RYC2019-027244-I) from the Spanish Ministry of Science and Innovation. Anna Esteve-Codina was funded by ISCIII /MINECO (PT17/0009/0019) and co-funded by FEDER. We acknowledge the support of the Spanish Ministerio de Economía y Competitividad for the “Severo Ochoa” Programme for Centres of Excellence in R&D (Project No. CEX2019-000902-S) to the Centre for Research in Agricultural Genomics (CRAG) and to the programmes of Centres de Recerca de Catalunya (CERCA).
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Nature Research
dc.relation.ispartof Sci Rep. 2023 Jan 11;13(1):535
dc.rights © The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.title Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1038/s41598-022-27016-x
dc.subject.keyword Fatty acids
dc.subject.keyword Gene expression
dc.subject.keyword Gene expression analysis
dc.subject.keyword Gene expression profiling
dc.subject.keyword Transcriptomics
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-82641-R
dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-112677RB-C22
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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