Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq

Mostra el registre complet Registre parcial de l'ítem

• dc.contributor.author Valdés-Hernández, Jesús
• dc.contributor.author Ramayo-Caldas, Yuliaxis
• dc.contributor.author Passols, Magí
• dc.contributor.author Sebastià, Cristina
• dc.contributor.author Criado-Mesas, Lourdes
• dc.contributor.author Crespo-Piazuelo, Daniel
• dc.contributor.author Esteve-Codina, Anna
• dc.contributor.author Castelló, Anna
• dc.contributor.author Sánchez, Armand
• dc.contributor.author Folch, Josep M.
• dc.date.accessioned 2023-03-09T07:22:17Z
• dc.date.available 2023-03-09T07:22:17Z
• dc.date.issued 2023
• dc.description.abstract Fatty acids (FAs) play an essential role as mediators of cell signaling and signal transduction, affecting metabolic homeostasis and determining meat quality in pigs. However, FAs are transformed by the action of several genes, such as those encoding desaturases and elongases of FAs in lipogenic tissues. The aim of the current work was to identify candidate genes, biological processes, and pathways involved in the modulation of intramuscular FA profile from longissimus dorsi muscle. FA profile by gas chromatography of methyl esters and gene expression by RNA-Seq were determined in 129 Iberian × Duroc backcrossed pigs. An association analysis between the muscle transcriptome and its FA profile was performed, followed by a concordance and functional analysis. Overall, a list of well-known (e.g., PLIN1, LEP, ELOVL6, SC5D, NCOA2, ACSL1, MDH1, LPL, LGALS12, TFRC, GOT1, and FBP1) and novel (e.g., TRARG1, TANK, ENSSSCG00000011196, and ENSSSCG00000038429) candidate genes was identified, either in association with specific or several FA traits. Likewise, several of these genes belong to biological processes and pathways linked to energy, lipid, and carbohydrate metabolism, which seem determinants in the modulation of FA compositions. This study can contribute to elucidate the complex relationship between gene expression and FA profile in pig muscle.
• dc.description.sponsorship This work was supported by the Spanish Ministerio de Ciencia e Innovación (MICINN) and the Fondo Europeo de Desarrollo Regional (FEDER) with project references: AGL2017-82641-R and PID2020-112677RB-C22. Jesús Valdés Hernández was funded with an FI PhD grant “Formació i contractació de personal investigador novell” fellowship provided by the “Agència de Gestió d’Ajuts Universitaris i de Recerca (AGAUR)” from Generalitat de Catalunya (2019FI_B_00787 credential). Yuliaxis Ramayo-Caldas was funded by a “Ramon y Cajal” contract (RYC2019-027244-I) from the Spanish Ministry of Science and Innovation. Anna Esteve-Codina was funded by ISCIII /MINECO (PT17/0009/0019) and co-funded by FEDER. We acknowledge the support of the Spanish Ministerio de Economía y Competitividad for the “Severo Ochoa” Programme for Centres of Excellence in R&D (Project No. CEX2019-000902-S) to the Centre for Research in Agricultural Genomics (CRAG) and to the programmes of Centres de Recerca de Catalunya (CERCA).
• dc.format.mimetype application/pdf
• dc.identifier.citation Valdés-Hernández J, Ramayo-Caldas Y, Passols M, Sebastià C, Criado-Mesas L, Crespo-Piazuelo D, Esteve-Codina A, Castelló A, Sánchez A, Folch JM. Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq. Sci Rep. 2023 Jan 11;13(1):535. DOI: 10.1038/s41598-022-27016-x
• dc.identifier.doi http://dx.doi.org/10.1038/s41598-022-27016-x
• dc.identifier.issn 2045-2322
• dc.identifier.uri http://hdl.handle.net/10230/56117
• dc.language.iso eng
• dc.publisher Nature Research
• dc.relation.ispartof Sci Rep. 2023 Jan 11;13(1):535
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/AGL2017-82641-R
• dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/PID2020-112677RB-C22
• dc.rights © The Author(s) 2023. Open Access This article is licensed under a Creative Commons Attribution 4.0 International License, which permits use, sharing, adaptation, distribution and reproduction in any medium or format, as long as you give appropriate credit to the original author(s) and the source, provide a link to the Creative Commons licence, and indicate if changes were made. The images or other third party material in this article are included in the article's Creative Commons licence, unless indicated otherwise in a credit line to the material. If material is not included in the article's Creative Commons licence and your intended use is not permitted by statutory regulation or exceeds the permitted use, you will need to obtain permission directly from the copyright holder. To view a copy of this licence, visit http://creativecommons.org/licenses/by/4.0/.
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.keyword Fatty acids
• dc.subject.keyword Gene expression
• dc.subject.keyword Gene expression analysis
• dc.subject.keyword Gene expression profiling
• dc.subject.keyword Transcriptomics
• dc.title Global analysis of the association between pig muscle fatty acid composition and gene expression using RNA-Seq
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion