SIMPLE: structured illumination based point localization estimator with enhanced precision
SIMPLE: structured illumination based point localization estimator with enhanced precision
Citació
- Reymond L, Ziegler J, Knapp C, Wang F, Huser T, Ruprecht V et al. SIMPLE: structured illumination based point localization estimator with enhanced precision. Opt Express. 2019 Aug 19;27(17):24578-24590. DOI:10.1364/OE.27.024578
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Resum
We present a structured illumination microscopy based point localization estimator (SIMPLE) that achieves a 2-fold increase in single molecule localization precision compared to conventional centroid estimation methods. SIMPLE advances the recently introduced MINFLUX concept by using precisely phase-shifted sinusoidal wave patterns as nanometric rulers for simultaneous particle localization based on photon count variation over a 20 μm field of view. We validate SIMPLE in silico and experimentally on a TIRF-SIM setup using a digital micro-mirror device (DMD) as a spatial light modulator.