SIMPLE: structured illumination based point localization estimator with enhanced precision
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- dc.contributor.author Reymond, Loïc
- dc.contributor.author Ziegler, Johannes
- dc.contributor.author Knapp, Christian
- dc.contributor.author Wang, Fung-Chen
- dc.contributor.author Huser, Thomas
- dc.contributor.author Ruprecht, Verena
- dc.contributor.author Wieser, Stefan
- dc.date.accessioned 2022-06-10T10:41:40Z
- dc.date.available 2022-06-10T10:41:40Z
- dc.date.issued 2019
- dc.description.abstract We present a structured illumination microscopy based point localization estimator (SIMPLE) that achieves a 2-fold increase in single molecule localization precision compared to conventional centroid estimation methods. SIMPLE advances the recently introduced MINFLUX concept by using precisely phase-shifted sinusoidal wave patterns as nanometric rulers for simultaneous particle localization based on photon count variation over a 20 μm field of view. We validate SIMPLE in silico and experimentally on a TIRF-SIM setup using a digital micro-mirror device (DMD) as a spatial light modulator.
- dc.description.sponsorship Funding: “la Caixa” Foundation (ID 100010434, LCF/BQ/IN18/11660032); Marie Skłodowska-Curie Horizon 2020 (713673, 754558, 642157); Spanish Ministry Centro de Excelencia Severo Ochoa (SEV-2015-0522); MINECO’s Plan Nacional (BFU2017-86296-P); Generalitat de Catalunya (CERCA); Fundació Privada Cellex
- dc.format.mimetype application/pdf
- dc.identifier.citation Reymond L, Ziegler J, Knapp C, Wang F, Huser T, Ruprecht V et al. SIMPLE: structured illumination based point localization estimator with enhanced precision. Opt Express. 2019 Aug 19;27(17):24578-24590. DOI:10.1364/OE.27.024578
- dc.identifier.doi http://dx.doi.org/10.1364/OE.27.024578
- dc.identifier.issn 1094-4087
- dc.identifier.uri http://hdl.handle.net/10230/53452
- dc.language.iso eng
- dc.publisher Optica
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/713673
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/754558
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/642157
- dc.relation.projectID info:eu-repo/grantAgreement/ES/2PE/BFU2017-86296-P
- dc.rights © 2019 Optical Society of America under the terms of the OSA Open Access Publishing Agreement
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.other Microscòpia
- dc.subject.other Cèl·lules
- dc.title SIMPLE: structured illumination based point localization estimator with enhanced precision
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion