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Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors

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dc.contributor.author Beltran Marqués, Antoni
dc.contributor.author Pahita, Elena
dc.contributor.author Ghosh, Subhanita
dc.contributor.author Lenhard, Boris
dc.contributor.author Sarkies, Peter
dc.date.accessioned 2022-04-27T10:57:58Z
dc.date.available 2022-04-27T10:57:58Z
dc.date.issued 2021
dc.identifier.citation Beltran T, Pahita E, Ghosh S, Lenhard B, Sarkies P. Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors. EMBO J. 2021 Mar 1;40(5):e105564. DOI:10.15252/embj.2020105564
dc.identifier.issn 0261-4189
dc.identifier.uri http://hdl.handle.net/10230/52913
dc.description.abstract Piwi-interacting RNAs (piRNAs) play key roles in germline development and genome defence in metazoans. In C. elegans, piRNAs are transcribed from > 15,000 discrete genomic loci by RNA polymerase II (Pol II), resulting in 28 nt short-capped piRNA precursors. Here, we investigate transcription termination at piRNA loci. We show that the Integrator complex, which terminates snRNA transcription, is recruited to piRNA loci. Moreover, we demonstrate that the catalytic activity of Integrator cleaves nascent capped piRNA precursors associated with promoter-proximal Pol II, resulting in termination of transcription. Loss of Integrator activity, however, does not result in transcriptional readthrough at the majority of piRNA loci. Taken together, our results draw new parallels between snRNA and piRNA biogenesis in nematodes and provide evidence of a role for the Integrator complex as a terminator of promoter-proximal RNA polymerase II during piRNA biogenesis.
dc.description.sponsorship We would like to thank Juan Cabello for kindly sharing JCP383 ints-6::GFP nematodes with us at the beginning of the study. This work was funded by the United Kingdom Medical Research Council (MC-A652-5PY80 “Epigenetics and Evolution”-PS; MC_UP_1102/1 “Computational Regulatory Genomics”- BL ) and by the Wellcome Trust (106954/Z/15/Z- BL)
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher EMBO Press
dc.rights © 2020 Toni Beltran et al. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited
dc.rights.uri http://creativecommons.org/licenses/by/4.0/
dc.subject.other RNAs associats a Piwi
dc.subject.other ARN no codificant
dc.subject.other Caenorhabditis elegans
dc.title Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.15252/embj.2020105564
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

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