Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors

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• dc.contributor.author Beltran Marqués, Antoni
• dc.contributor.author Pahita, Elena
• dc.contributor.author Ghosh, Subhanita
• dc.contributor.author Lenhard, Boris
• dc.contributor.author Sarkies, Peter
• dc.date.accessioned 2022-04-27T10:57:58Z
• dc.date.available 2022-04-27T10:57:58Z
• dc.date.issued 2021
• dc.description.abstract Piwi-interacting RNAs (piRNAs) play key roles in germline development and genome defence in metazoans. In C. elegans, piRNAs are transcribed from > 15,000 discrete genomic loci by RNA polymerase II (Pol II), resulting in 28 nt short-capped piRNA precursors. Here, we investigate transcription termination at piRNA loci. We show that the Integrator complex, which terminates snRNA transcription, is recruited to piRNA loci. Moreover, we demonstrate that the catalytic activity of Integrator cleaves nascent capped piRNA precursors associated with promoter-proximal Pol II, resulting in termination of transcription. Loss of Integrator activity, however, does not result in transcriptional readthrough at the majority of piRNA loci. Taken together, our results draw new parallels between snRNA and piRNA biogenesis in nematodes and provide evidence of a role for the Integrator complex as a terminator of promoter-proximal RNA polymerase II during piRNA biogenesis.
• dc.description.sponsorship We would like to thank Juan Cabello for kindly sharing JCP383 ints-6::GFP nematodes with us at the beginning of the study. This work was funded by the United Kingdom Medical Research Council (MC-A652-5PY80 “Epigenetics and Evolution”-PS; MC_UP_1102/1 “Computational Regulatory Genomics”- BL ) and by the Wellcome Trust (106954/Z/15/Z- BL)
• dc.format.mimetype application/pdf
• dc.identifier.citation Beltran T, Pahita E, Ghosh S, Lenhard B, Sarkies P. Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors. EMBO J. 2021 Mar 1;40(5):e105564. DOI:10.15252/embj.2020105564
• dc.identifier.doi http://dx.doi.org/10.15252/embj.2020105564
• dc.identifier.issn 0261-4189
• dc.identifier.uri http://hdl.handle.net/10230/52913
• dc.language.iso eng
• dc.publisher EMBO Press
• dc.rights © 2020 Toni Beltran et al. This is an open access article under the terms of the http://creativecommons.org/licenses/by/4.0/ License, which permits use, distribution and reproduction in any medium, provided the original work is properly cited
• dc.rights.accessRights info:eu-repo/semantics/openAccess
• dc.rights.uri http://creativecommons.org/licenses/by/4.0/
• dc.subject.other RNAs associats a Piwi
• dc.subject.other ARN no codificant
• dc.subject.other Caenorhabditis elegans
• dc.title Integrator is recruited to promoter-proximally paused RNA Pol II to generate Caenorhabditis elegans piRNA precursors
• dc.type info:eu-repo/semantics/article
• dc.type.version info:eu-repo/semantics/publishedVersion