Welcome to the UPF Digital Repository

Widespread ribosome stalling in a genome-reduced bacterium and the need for translational quality control

Show simple item record

dc.contributor.author Burgos, Raul
dc.contributor.author Weber, Marc
dc.contributor.author Gallo López, Carolina, 1984-
dc.contributor.author Lluch-Senar, Maria 1982-
dc.contributor.author Serrano Pubull, Luis, 1982-
dc.date.accessioned 2022-01-24T12:21:33Z
dc.date.available 2022-01-24T12:21:33Z
dc.date.issued 2021
dc.identifier.citation Burgos R, Weber M, Gallo C, Lluch-Senar M, Serrano L. Widespread ribosome stalling in a genome-reduced bacterium and the need for translational quality control. iScience. 2021 Aug 16;24(9):102985. DOI: 10.1016/j.isci.2021.102985
dc.identifier.issn 2589-0042
dc.identifier.uri http://hdl.handle.net/10230/52298
dc.description.abstract Trans-translation is a ubiquitous bacterial mechanism of ribosome rescue mediated by a transfer-messenger RNA (tmRNA) that adds a degradation tag to the truncated nascent polypeptide. Here, we characterize this quality control system in a genome-reduced bacterium, Mycoplasma pneumoniae (MPN), and perform a comparative analysis of protein quality control components in slow and fast-growing prokaryotes. We show in vivo that in MPN the sole quality control cytoplasmic protease (Lon) degrades efficiently tmRNA-tagged proteins. Analysis of tmRNA-mutants encoding a tag resistant to proteolysis reveals extensive tagging activity under normal growth. Unlike knockout strains, these mutants are viable demonstrating the requirement of tmRNA-mediated ribosome recycling. Chaperone and Lon steady-state levels maintain proteostasis in these mutants suggesting a model in which co-evolution of Lon and their substrates offer simple mechanisms of regulation without specialized degradation machineries. Finally, comparative analysis shows relative increase in Lon/Chaperone levels in slow-growing bacteria suggesting physiological adaptation to growth demand.
dc.description.sponsorship This work has been supported by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program under grant agreement 670216 (MYCOCHASSIS), and Conveni La Caixa 2020–2023 (LA CAIXA-EGA, LCF/PR/GN13/10260009). We also acknowledge the support of the Spanish Ministry of Science and Innovation to the EMBL partnership, the Centro de Excelencia Severo Ochoa, and the CERCA Program from the Generalitat de Catalunya. M.W acknowledges the European Union's Horizon 2020 research and innovation program under grant agreement 634942 (MycoSynVac). The proteomics analyses were performed in the CRG/UPF Proteomics Unit which is part of the Proteored, PRB3 and is supported by grant PT17/0019, of the PE I + D + i 2013–2016, funded by ISCIII and ERDF
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.rights © 2021 Raul Burgos et al. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
dc.subject.other Genètica
dc.subject.other Genòmica
dc.subject.other Bacteriologia
dc.subject.other Proteïnes
dc.title Widespread ribosome stalling in a genome-reduced bacterium and the need for translational quality control
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.isci.2021.102985
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/670216
dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634942
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/publishedVersion

Thumbnail

This item appears in the following Collection(s)

Show simple item record

Search DSpace


Advanced Search

Browse

My Account

Statistics

Compliant to Partaking