Widespread ribosome stalling in a genome-reduced bacterium and the need for translational quality control
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- dc.contributor.author Burgos, Raul
- dc.contributor.author Weber, Marc
- dc.contributor.author Gallo López, Carolina, 1984-
- dc.contributor.author Lluch-Senar, Maria 1982-
- dc.contributor.author Serrano Pubull, Luis, 1982-
- dc.date.accessioned 2022-01-24T12:21:33Z
- dc.date.available 2022-01-24T12:21:33Z
- dc.date.issued 2021
- dc.description.abstract Trans-translation is a ubiquitous bacterial mechanism of ribosome rescue mediated by a transfer-messenger RNA (tmRNA) that adds a degradation tag to the truncated nascent polypeptide. Here, we characterize this quality control system in a genome-reduced bacterium, Mycoplasma pneumoniae (MPN), and perform a comparative analysis of protein quality control components in slow and fast-growing prokaryotes. We show in vivo that in MPN the sole quality control cytoplasmic protease (Lon) degrades efficiently tmRNA-tagged proteins. Analysis of tmRNA-mutants encoding a tag resistant to proteolysis reveals extensive tagging activity under normal growth. Unlike knockout strains, these mutants are viable demonstrating the requirement of tmRNA-mediated ribosome recycling. Chaperone and Lon steady-state levels maintain proteostasis in these mutants suggesting a model in which co-evolution of Lon and their substrates offer simple mechanisms of regulation without specialized degradation machineries. Finally, comparative analysis shows relative increase in Lon/Chaperone levels in slow-growing bacteria suggesting physiological adaptation to growth demand.
- dc.description.sponsorship This work has been supported by the European Research Council (ERC) under the European Union's Horizon 2020 research and innovation program under grant agreement 670216 (MYCOCHASSIS), and Conveni La Caixa 2020–2023 (LA CAIXA-EGA, LCF/PR/GN13/10260009). We also acknowledge the support of the Spanish Ministry of Science and Innovation to the EMBL partnership, the Centro de Excelencia Severo Ochoa, and the CERCA Program from the Generalitat de Catalunya. M.W acknowledges the European Union's Horizon 2020 research and innovation program under grant agreement 634942 (MycoSynVac). The proteomics analyses were performed in the CRG/UPF Proteomics Unit which is part of the Proteored, PRB3 and is supported by grant PT17/0019, of the PE I + D + i 2013–2016, funded by ISCIII and ERDF
- dc.format.mimetype application/pdf
- dc.identifier.citation Burgos R, Weber M, Gallo C, Lluch-Senar M, Serrano L. Widespread ribosome stalling in a genome-reduced bacterium and the need for translational quality control. iScience. 2021 Aug 16;24(9):102985. DOI: 10.1016/j.isci.2021.102985
- dc.identifier.doi http://dx.doi.org/10.1016/j.isci.2021.102985
- dc.identifier.issn 2589-0042
- dc.identifier.uri http://hdl.handle.net/10230/52298
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/670216
- dc.relation.projectID info:eu-repo/grantAgreement/EC/H2020/634942
- dc.rights © 2021 Raul Burgos et al. This is an open access article under the CC BY-NC-ND license (http://creativecommons.org/licenses/by-nc-nd/4.0/)
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.rights.uri http://creativecommons.org/licenses/by-nc-nd/4.0/
- dc.subject.other Genètica
- dc.subject.other Genòmica
- dc.subject.other Bacteriologia
- dc.subject.other Proteïnes
- dc.title Widespread ribosome stalling in a genome-reduced bacterium and the need for translational quality control
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/publishedVersion