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Transcription-mediated supercoiling regulates genome folding and loop formation

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dc.contributor.author Neguembor, Maria Victoria
dc.contributor.author Martin, Laura
dc.contributor.author Castells García, Àlvaro, 1991-
dc.contributor.author Gómez García, Pablo
dc.contributor.author Vicario, Chiara
dc.contributor.author Carnevali, Davide
dc.contributor.author Abed, Jumana AlHaj
dc.contributor.author Granados, Alba
dc.contributor.author Sebastián Pérez, Rubén
dc.contributor.author Sottile, Francesco, 1988-
dc.contributor.author Solon, Jérôme
dc.contributor.author Wu, Chao-Ting
dc.contributor.author Lakadamyali, Melike
dc.contributor.author Cosma, Maria Pia, 1970-
dc.date.accessioned 2021-09-10T07:19:02Z
dc.date.issued 2021
dc.identifier.citation Neguembor MV, Martin L, Castells-García Á, Gómez-García PA, Vicario C, Carnevali D, AlHaj Abed J, Granados A, Sebastian-Perez R, Sottile F, Solon J, Wu CT, Lakadamyali M, Cosma MP. Transcription-mediated supercoiling regulates genome folding and loop formation. Mol Cell. 2021;81(15):3065-81.e12. DOI: 10.1016/j.molcel.2021.06.009
dc.identifier.issn 1097-2765
dc.identifier.uri http://hdl.handle.net/10230/48422
dc.description.abstract The chromatin fiber folds into loops, but the mechanisms controlling loop extrusion are still poorly understood. Using super-resolution microscopy, we visualize that loops in intact nuclei are formed by a scaffold of cohesin complexes from which the DNA protrudes. RNA polymerase II decorates the top of the loops and is physically segregated from cohesin. Augmented looping upon increased loading of cohesin on chromosomes causes disruption of Lamin at the nuclear rim and chromatin blending, a homogeneous distribution of chromatin within the nucleus. Altering supercoiling via either transcription or topoisomerase inhibition counteracts chromatin blending, increases chromatin condensation, disrupts loop formation, and leads to altered cohesin distribution and mobility on chromatin. Overall, negative supercoiling generated by transcription is an important regulator of loop formation in vivo.
dc.format.mimetype application/pdf
dc.language.iso eng
dc.publisher Elsevier
dc.relation.ispartof Mol Cell. 2021;81(15):3065-81.e12
dc.rights © Elsevier http://dx.doi.org/10.1016/j.molcel.2021.06.009
dc.title Transcription-mediated supercoiling regulates genome folding and loop formation
dc.type info:eu-repo/semantics/article
dc.identifier.doi http://dx.doi.org/10.1016/j.molcel.2021.06.009
dc.subject.keyword STORM microscopy
dc.subject.keyword Cohesin
dc.subject.keyword Genome folding
dc.subject.keyword Super-resolution microscopy
dc.subject.keyword Supercoiling
dc.subject.keyword Transcription
dc.rights.accessRights info:eu-repo/semantics/openAccess
dc.type.version info:eu-repo/semantics/acceptedVersion


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