Transcription-mediated supercoiling regulates genome folding and loop formation
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- dc.contributor.author Neguembor, Maria Victoria
- dc.contributor.author Martin, Laura
- dc.contributor.author Castells García, Àlvaro, 1991-
- dc.contributor.author Gómez García, Pablo
- dc.contributor.author Vicario, Chiara
- dc.contributor.author Carnevali, Davide
- dc.contributor.author Abed, Jumana AlHaj
- dc.contributor.author Granados, Alba
- dc.contributor.author Sebastián Pérez, Rubén
- dc.contributor.author Sottile, Francesco, 1988-
- dc.contributor.author Solon, Jérôme
- dc.contributor.author Wu, Chao-Ting
- dc.contributor.author Lakadamyali, Melike
- dc.contributor.author Cosma, Maria Pia, 1970-
- dc.date.accessioned 2021-09-10T07:19:02Z
- dc.date.issued 2021
- dc.description.abstract The chromatin fiber folds into loops, but the mechanisms controlling loop extrusion are still poorly understood. Using super-resolution microscopy, we visualize that loops in intact nuclei are formed by a scaffold of cohesin complexes from which the DNA protrudes. RNA polymerase II decorates the top of the loops and is physically segregated from cohesin. Augmented looping upon increased loading of cohesin on chromosomes causes disruption of Lamin at the nuclear rim and chromatin blending, a homogeneous distribution of chromatin within the nucleus. Altering supercoiling via either transcription or topoisomerase inhibition counteracts chromatin blending, increases chromatin condensation, disrupts loop formation, and leads to altered cohesin distribution and mobility on chromatin. Overall, negative supercoiling generated by transcription is an important regulator of loop formation in vivo.
- dc.format.mimetype application/pdf
- dc.identifier.citation Neguembor MV, Martin L, Castells-García Á, Gómez-García PA, Vicario C, Carnevali D, AlHaj Abed J, Granados A, Sebastian-Perez R, Sottile F, Solon J, Wu CT, Lakadamyali M, Cosma MP. Transcription-mediated supercoiling regulates genome folding and loop formation. Mol Cell. 2021;81(15):3065-81.e12. DOI: 10.1016/j.molcel.2021.06.009
- dc.identifier.doi http://dx.doi.org/10.1016/j.molcel.2021.06.009
- dc.identifier.issn 1097-2765
- dc.identifier.uri http://hdl.handle.net/10230/48422
- dc.language.iso eng
- dc.publisher Elsevier
- dc.relation.ispartof Mol Cell. 2021;81(15):3065-81.e12
- dc.rights © Elsevier http://dx.doi.org/10.1016/j.molcel.2021.06.009
- dc.rights.accessRights info:eu-repo/semantics/openAccess
- dc.subject.keyword STORM microscopy
- dc.subject.keyword Cohesin
- dc.subject.keyword Genome folding
- dc.subject.keyword Super-resolution microscopy
- dc.subject.keyword Supercoiling
- dc.subject.keyword Transcription
- dc.title Transcription-mediated supercoiling regulates genome folding and loop formation
- dc.type info:eu-repo/semantics/article
- dc.type.version info:eu-repo/semantics/acceptedVersion